PPARγ-mTORC2通路介导黄绿青霉素所致细胞自噬的作用研究

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1、密级硕士学位论文＃ＰＰＡＲ－ｍＴＯＲＣ２通路介导黄绿青霉素？所致细胞自噬的作用研究作者姓名：冯敞指导教师：陈敏学科专业：营养与食品卫生学大连医科大学目录一、摘要........................................................................................................1（一）中文摘要...............................................................

2、..........................1（二）英文摘要.........................................................................................3二、正文........................................................................................................6（一）前言...................................

3、..............................................................6（二）材料和方法.....................................................................................71.材料.....................................................................................................71.1实验对象...

4、........................................................................................71.2药品和主要试剂................................................................................71.3主要仪器......................................................................................

5、.....82.方法.....................................................................................................92.1实验动物饲养与处理.......................................................................92.2细胞培养、处理及染毒................................................................

6、...92.3MTT实验...........................................................................................92.4HE染色............................................................................................102.5蛋白质印迹法（Westernblot）..............................................

7、......102.6反转录实时定量PCR（RT-qPCR）..............................................132.7酶联免疫吸附测定（ELISA）.....................................................152.8PPAR转录因子的测定...................................................................152.9统计方法...............................

8、..........................................................16（三）结果........................