妊高征胎盘绒毛组织内皮素1及其受体的mRNA表达

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1、硕士学位论文摘要妊高征胎盘绒毛组织内皮素一1及其受体的mRNA表达摘要目的：检测妊高征患者及正常妊娠产妇胎盘绒毛组织内皮素一1(ET一1)及其受体(ETA、ETB)的mRNA表达，探讨其与妊高征的关系。方法：60例妊高征患者(妊高征组)和30例正常妊娠孕妇(正常妊娠组)，在剖宫产术中取胎盘绒毛组织，采用逆转录聚合酶链反应(RT—PCR)检测绒毛组织ET—l、ETA和ETB的mRNA表达，并设立GAPDH内对照对PCR产物作半定量分析。结果：胎盘绒毛组织存在ET一1、ETA及ETBmRNA的表达，且妊高征组ET—l和ETAmRNA表达均明显高于正常对照组，两组间差

2、异有显著性(P均

3、ETB作用的不同，ETA、ETB平衡的失调亦可能参与妊高征的发病。关键词妊娠并发症，高血压，胎盘，ET一1，ETA，ETB，RNA，信使硕士学位论文摘要Expressionofendothelin-IandendothelinreceptorsmRNAinplacentalvillousfrompregnancyinducedhypertensionABSTRACTobjective：TodetectthemRNAexpressionofendothelin-I(ET-1)，ET-lreceptorsE1’AandETBinplacentavillousfrom

4、normalpregnancy(ro)andpregnancyinducedhypertension(PIH)．Andtoinvestigatetheroleofendothelin·1andendothelinreceptorsinthepathogenesisofpregnancyinducedhypertension．Methods：ThemRNAlevelsofET-1，ETAandETBweredeterminedusingRT-PCRandexpressedasarbitraryunitsaftercorrectionforcontrolGAPDHg

5、enemRNAlevelsinplacentavillousfrom30NPand60Pm．Results：ThemRNAexpressionofET-1，ETAandETBwerefoundinplacentafrombethPIHandNPThemRNAlevelsofET-1andETAweresignificantlyhigherinPIHthanthatinNP(p

6、TBwerefoundinplacenta．Theplacentaisshortofnerveregulationitself,SOweconsiderETasaparacrinefactorplaysallimportantroleinfetal-placentalcirculation．ET--ETRsystemmayparticipateinthenormaldevelopmentofplacentaandtheregulationofthefetal—placentMbloodvessels．2．肌lenET一1iscombinedwithETA．itc

7、anarose-intensevesselcontraction。BecausetheexpressionofET-IandETAmRNAwerebothsignificantlyhigherinplacentaofPIH，theresistanceoffetal．placentalcirculationwasincreased．and也eplacentawasshortofbloodandoxygen，andtheplacentamayreleaseaseriesoftoxicityfactors．Wbconsiderthismaybeoneofthereas

8、onsthatcause