放射线治疗包虫病的实验研究

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1、兰州人学硕匕学位论文放射线治疗包虫病的实验研究摘要目的：通过观察不同剂量6MVX线照射对大鼠继发性棘球蚴活性的影响，探讨放射线治疗棘球蚴病的可行性及其机制。方法：对从大鼠体内分离出的棘球蚴囊分别以lO、20、30和40Gy的6MVX线进行照射后，观察棘球蚴囊的超微结构，测定囊液中总蛋白和钙离子含量，并通过草酸豁一焦锑酸盐细胞化学法(PPA)探讨放射线治疗包虫病的机理。结果：与空白对照组相比，各实验组棘球蚴囊超微结构均可见不同程度的改变和破坏；囊液总蛋白含量明显降低而钙离子含量明显增高，且以高剂量组更为显著。PPA法显示实验组内质网与线粒体扩张且膜上可见黑色光滑

2、的钙盐沉积。结论：6MvX线对棘球蚴囊有一定破坏作用，且在一定的范围内存在量效关系。关键词：棘球蚴病细粒棘球绦虫放射治疗6MVX线体外培养草酸盐一焦锑酸盐细胞化学法大鼠兰州大学硕l：学位论文T1leExperimentalStudyofradiotherapyonEchinococcosisAbstractObjective：Todemonstratethepossibilityandmechanismofradiotherapybyinvestigatingtheeffectsofdifferentdosesof6MVX—rayradiotherapyon点

3、’cJIl伽DcDccMsg，．口，l“肠5Hsinrat．Methods：AnerbeingirradiatedbylO，20，30and40GyOf6MVX—ray，theseexaminationswerecarriedoutsuchastheultrastructureofthe上_c，i切DcDcc埘gr口，l“幻s“scystsinratwithelectron—microscope，thetotalproteinamountinhydatidcystfluid(HCF)andCa2+ioninHCF．Thepotassium—pyroantimo

4、nate(PPA)cytochemicalmethodwasusedtOdemonstratewhetherblOckedcalCiumchannelswouldbe0nereasonofradiotherapy0n眈JIl觑DcoccHsgr口，l“肠s“scystsinrat．Results：ThecystwaUtissueandceUsappearedabnonnalchangesanddamagesmicroscopicallyinthetreatedgroupstoadifferentextent．ThetOtalproteinamountinhyd

5、atidcystnuid(HCF)increased，whileCa2+inHCFreducedobViouslyinthetfeatedgroups，especiallyinhighdosegrOups．WithPPA，someelectron。densepfecipitateswerepresentedonthemitochondriaandendOcytoplasmicreticuluminthetreated伊oups．Conclusion：ThestmctureofthecystsofR，i讥DcDcc“5g，．口咒“幻s“sinratcanbeda

6、magedbyradiotherapywhichshowedaquantity—efficiencyrelationtosomeextent．Keywords：Cysticechinococcosis；Ec^加ocDcc“sg，．口，l“肠s“5；Radiotherapy；6MVX—ray；CultufeinVitro；Potassium-pyroantimonate；RatII兰州人学硕t：学位论义CECysticechinococcosisABZAlbendazoleMBZMebendazolePTprecisiOnradiotherapyPTVplann

7、ingtargetVolumeSIBNBCrbiolo西caltargeVolume缩略词表囊型包虫病阿苯达唑甲苯咪唑精确放疗计划靶区生物靶区simultaneouslyinte伊atedboosting追加剂量照射Boronneutroncapturetherapy硼中子俘获治疗系统highlinearenergytransferPETpositronemissiontomographyPBSphosphatebufferedsolutionPPAPhosphate-Pyroantimonate38高线性能量转移正电子发射断层磷酸盐缓冲液磷酸盐—焦锑酸盐细胞

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