棉花细胞质雄性不育系线粒体基因组细菌人工染色体文库的构建及其鉴定

《棉花细胞质雄性不育系线粒体基因组细菌人工染色体文库的构建及其鉴定》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、新疆农业大学硕士学位论文棉花细胞质雄性不育系线粒体基因组细菌人工染色体文库的构建及其鉴定姓名：代培红申请学位级别：硕士专业：分子生物学与生物化学指导教师：郭三堆;罗淑萍20070601AbstractThecoltonisoneofthemajnindustfjalcropSinoufcounlrHWilhIbefextileindustryandtheJjvesofthepeople1evelenhancement，ChinaalTeadybecomethebiggestcotto“producercountryandelpense

2、countryintheworId．Wilhincre船ingunceasin91yt0cotcon毫demand，mordertomeetpeople、口eed，出ecullivationofhi91lproduction，thehighqualitycottonnewVarietytos01Vet11equestionis她incvitablewayNow，t11eworldcottonbrcedingmainresearchdirectionusillgthecottoncytoplasmicm“e—slerilelinecuI

3、livat抽nco【tonhybridvar主ecy．Themassiveresearchindicatethatoytoplasmicmalesterilityhas111ecloserelationwi也tllemitochondr瑚DNAvariation．Therefore，inordertofesearchoytoplasmicmalesterilitywifhmjtochond“alDNAbetweenreJationsfurfheL出isrese3rchwilicons￡『uctcofc()nmifo曲ondfialDN

4、Abacterialan琦cialchromosome1mrary，andhascarricdonthepreliminaryappraisaltothelib豫ry，malesterilitybundsthesolidfounda【ionfofamorethorougbunderstandi“gandcxplana60ncytopIa5mw“hIhemitochonddonbetweenrelations．This陀searchtoketlleuplandcotton(Gosl)pf啪^批，Hmm)cytophsmicmale·st

5、erilelineP30Ae￡joJa￡ionseed】jngasthe脚ate“aj．Refe佗ncence，maizeandoth盯cfops’Jl】nocbondrialDNAbactedalanificialchromosomelibrafyconstmctionmethods，加duⅡifiescottonitselfloindudemegossipol，tannineasnytoox埘jzcmateriaIs’parlicularity，estabJishedah远hquaIj‘ymitochondfiaJDNA’sBAc

6、librafysystem．nissysI锄containedthreeveryimportantcondjtions：thehighmolecularweighImtDNAexIraction，dephosphorizationeffectgoodcarriefpreparation，transfonnede茌iciencyh嘞electroco叩tencellpreparation．1nth。processofs8paratingmitochoⅡ“onanditsDNA，fhemjtochondrjonwhich曲taiⅡedus

7、ingdiscontinuoussucmscgradjen￡cenfr，fugation，afler曲e玎uoroscopeexaminationjndjcafedfhaficsjntc鲥￡ywasgood，didnothaveponutionssuchaschloroplasIandceUnucleusandsoon．Weobta．medmi【ochondrialDNAusingtheembeddingdecompositionmethod．AndexamineIh。pur“yusiogthedigestingwith月mmⅡand

8、丑n小HI，PCRapplifiction．TheresultsindicatedIhatlhemitochondria】DNApuritywashigh，thequalitywasgo。d；thedeph05ph。ri