Cbfa1基因修饰间充质干细胞治疗骨缺损的实验研究

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1、第三军医大学博士学位论文英文缩写一览表英文缩写英文全称中文全称AdAdenovirus腺病毒Osf2Osteobalstspecificalfactor2成骨特异性转录因子2CbfalCorebindingfactoral核心连接蛋白a1CMVcytomegalovirus巨细胞病毒CPEcytopathiceffect细胞病变LBLuriabrothLuria肉汤培养基DEPcdiethylpyrocarbonate焦二酸二乙酯MoImultiplicityofinfection感染复数PFUplaqueformingunit空斑形成单位X-gal5-bromo-4--chloro-3-

2、ladolyl··5一溴_4一氯一3一吲哚B-D—galactoside一13一D一半乳糖苷NTPribonuclesidetriophosPhate三磷酸核苷酸Rnaseribonuclease核糖核酸酶ODopticaldensity光密度mRNAmessengerRNA信使核糖核酸KDkilo—dillton千道尔顿Bpbasepair碱基对BSABovinesenlmalbumin牛血清白蛋白SDSsodiumdodecylsulfonate十二烷基磺酸钠EDTAethylenedianimetetraaceticacid乙二胺四乙酸OCNosteoca__Icin骨钙素ALPal

3、kalinephosphatase碱性磷酸酶OPNosteopontin骨桥蛋白BSPbonesialoprotein骨涎蛋白RT—PCRreversetranscription—polymerase逆转录．多聚酶链反应DGGEdenaturinggradientgelelectrophoresis变性梯度凝胶电泳PAGEpolyacrylamidegelelectrophoresis聚丙烯酰胺凝胶电泳1TAEDABDTTMSCsFCSDMSoGFPECMOB第三军医大学博士学位论文Tris·aceticacidandEDTAbufferTrisdiaminobezidindithioth

4、reitolmesenchymalstemcellsFetalCalfSerumdimethylsulfoxidegreenfluorescentproteinextracellularmatrixprotein0steoblast2乙酸乙二胺四乙酸缓冲液二氨基联苯胺二硫苏糖醇间充质干细胞胎牛血清二甲基亚砜绿色荧光蛋白骨细胞外基质蛋白成骨细胞第三军医大学博士学位论文ExperimentalstudyofCbfalgeneticallyModifiedMSCsrepairtherabbitbonedefectAbstractBonedefectresultingfromvarioustrau

5、mainfectionandtumorresectionisadi砸cultprobleminorthopaedicsfield．AutologousbonegraftisconsideredtObethegoldstandardofbonegraftmaterial，butthereisonlyalimitedquantityofboneavailabletoharvest．Moreover,harvestingofautologousbonegraftshassignificantmorbidity,includingpersistentdonorsitepainandlocalinf

6、ection。Allogenicbonegraftisanotheralternativetotheautograft．However，thereisriskofpossiblerejectionandpotentialpathogenstransmission．From1990s，investigatorshavepaidmoreattentiontoexploitandresearchthetissueengineeringbone．Ingeneral，theseedingcellsculturedinvitroareseededinnativeorsyntheticextracell

7、ularmatrixwhichcouldbebiocompatibleandbiodegradable，andthecomplexofseedingcellswithscaffoldwasimplantedinthebodyforexpectingcelleventssuchasadhesion，proliferation，differentiationandSOon．Atpresent，thefocusofboneti