NEB酶切buffer

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1、Home>TechnicalReference>RestrictionEndonucleases>NEBufferActivityChartNEBufferActivityChartforRestrictionEnzymesForyourconvenience,NewEnglandBiolabsoffersasimple4buffersystem.Acolor-coded10XNEBufferissuppliedwitheveryrestrictionendonuclease,ensuring100%activit

2、y.Over170restrictionenzymesexhibit100%activityinNEBuffer4,resultinginincreasedefficiency,flexibilityandeaseofuseespeciallywhenperformingdoubledigests.Tohelpselectthebestconditionsfordoubledigests,thischartshowstheoptimal(supplied)NEBufferandapproximateactivity

3、inthefourstandardNEBuffersandNEBufferEcoRIforeachenzyme.IfBSAissuppliedwithanenzyme,itisincludedinallNEBufferactivityreactions.Inaddition,thetableshowsrecommendedreactiontemperature,heatinactivationtemperature,recommendeddiluentbufferandwhethertheenzymeisTime-

4、Saverqualified(i.e.,cleavessubstratein5minutesunderrecommendedconditions).Note:Thevalueslistedinthistableareapproximate.Theywereobtainedusingeachenzyme'sspecificunitassaysubstrateDNA.Pleasecheckoutothertechnicalreferenceinformationrelatedtorestrictionenzymes:D

5、oubleDigestion

6、HeatInactivation

7、Activityat37°C

8、DiluentBuffers

9、Time-SaverEnzymes

10、HighFidelity(HF)RestrictionEnzymesClickfortheChartLegend,IconDescriptionA

11、B

12、C

13、D

14、E

15、F

16、H

17、I

18、K

19、L

20、M

21、N

22、P

23、R

24、S

25、T

26、X

27、Z

28、EnzymeSuppliedNEBuffer%ActivityinNEBuffersHeatInac.°CIncu.Temp.°CDilu-en

29、t1234EcoRIAatIINEBuffer405050100256537AAcc65INEBuffer3+BSA107510025506537AAccINEBuffer450501010058037AAciINEBuffer32550100501006537AAclINEBuffer4+BSA101001005No37BAcuINEBuffer4+SAM50100501001006537BAfeINEBuffer42550251001006537BAflIINEBuffer4+BSA50100251000653

30、7AAflIIINEBuffer3+BSA2575100501008037BAgeINEBuffer1100501075506537CAgeI-HF™NEBuffer4+BSA1005010100256537AAgeI-HF™RE-Mix™-NRNRNRNR-6537-AhdINEBuffer4+BSA25750100106537AAleINEBuffer410251010006537AAluINEBuffer4100100751001006537BAlwINEBuffer4501001010056537AAlwN

31、INEBuffer410100100100106537AApaINEBuffer4+BSA2550010056525AApaLINEBuffer4+BSA100100101005No37AApeKINEBuffer3257510050100No75BApoINEBuffer3+BSA1075100751008050AAscINEBuffer401010100