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1、华西口腔医学杂志第26卷第2期2008年4月WestChinaJournalofStomatologyVol.26No.2Apr.2008·211·[文章编号]1000-1182(2008)02-0211-04丝素蛋白/纳米羟磷灰石支架的实验研究1211赵勇,李罡,陈静,陈治清（1.口腔疾病研究国家重点实验室,四川大学,四川成都610041;2.昆明医学院口腔医院修复科,云南昆明650031)[摘要]目的设计和构建三维丝素蛋白/羟磷灰石骨组织工程支架材料。方法联合运用丝素蛋白非编织方法和仿生矿化技术，制备并表征三维多孔丝素蛋白/纳米羟磷灰石的有机/无机组织工程支架。结果仿生矿化在非编织支架上
2、形成的针状羟磷灰石晶体，直径20～60nm，长100～300nm。复合支架孔隙度为70%～78%，孔径为（163.4±42.6）μm。结论采用非编织丝素蛋白和仿生矿化的方法可制备孔隙度和孔径可控的组织工程支架。[关键词]丝素蛋白；仿生矿化；羟磷灰石[中图分类号]R783.1[文献标识码]A1Preparationandcharacteristicsofnon-wovensilkfibroin/nano-hydroxyapatitescaffoldsZHAOYong，LI211Gang,CHENJing,CHENZhi-qing.（1.StateKeyLaboratoryofOralDisea
3、ses,SichuanUniversity,Chengdu610041,China;2.Dept.ofProsthodontics,StomatologicalHospitalofKunmingMedicalCollege,Kunming650031,China）[Abstract]ObjectiveTheaimofthepresentstudywastodesignandfabricateathreedimensiona（l3D）porousstructureofsilkfibroin/apatiteusedasapotentialscaffoldinbonetissueengineeri
4、ng.MethodsWiththecombininguseofnon-wovensilkfibroinnetandbiomimeticmethod,porousnon-wovensilkfibroin/nano-hydroxyapatitenet（NSF/nHAP）waspreparedandcharacterizedwithX-raydiffraction（XRD）,scanningelectronmicroscope（SEM）andfouriertransforminfraredspectroscopy（FTIR）.Theporosityandswellingratioofthe3Dsc
5、affoldwerealsomeasured.Besides,theosteoblastsfromthecraniumofnewbornSDratwereculturedonthepre-fabricatedscaffoldtoevaluatethebiologicalreactionofthescaffold.ResultsThenano-sizedhydroxyapatitecrystalswereneedle-likewiththelengthof100-300nmandthediameterof20-60nm.Thescaffoldfabricatedinthepresentstud
6、yexhibitedtheporousmicrostructurewithopenporosityaround70%-78%.Itsaverageporesizewasabou（t163.4±42.6）μm.Theswellingratioandwateruptakingwere4.56%and81.93%,respectively,whichrevealedthatthe3Dporousscaffoldhadanexcellenthydrophilicity.Therod-shapedapatitecrystalscouldrapidlyformonthesurfaceoffibroinf
7、ibersthroughoutthenetworkbyimmersingthenetintocalciumandphosphatesolutionsalternatively.ConclusionA3DporousNSF/nHAPscaffoldcanbefabricatedbybiomimeticmineralizationandnone-wovensilkfibroinmethod.ThenovelNSF
