丝素蛋白膜的微纳米图案化与应用

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1、ThePreparationandapplicationofMicro-andNanopatternedSilkFibroinFilmsAbstractThePreparationandApplicationofMicro-andNanopatternedSilkFibroinFilmsAbstractBiomaterialsfortissueengineeringmustdemonstrateseveralcriticalfeaturesforpotentialutilityinvivo,includingnon-immunogenicresponse,

2、excellentmechanicalproperties,biocompatibilityandcontrollabledegradationrates.Silkfilmbiomaterialsweredesignedandcharacterizedtomeetthesefunctionalrequirements.Theuniquecombinationofelasticityandstrengthalongwithmammaliancellcompatibilitymakessilkfibroinanattractivematerialfortiss

3、ueengineering.Inaddition,silkfilmsurfacescanbepatternedusingsoftlithographictechniquestoproducehighresolutionsurfacefeatures.Inthefieldsofbiomaterialsandtissueengineering,itisofgreatimportancetostudytheeffectsofsurfacetopographyoncellbehavior.Weusedsimplesoftprintingtechniquessucc

4、essfullyobtainedavarietyofmicroandnano-patternedsilkfibroinmembrane,atthesametimethesilkproteinischemicallymodifiedandeventuallywontwotypesofsilkproteinmembranetopologywithgeometricpatterningandchemicalpatterning.Consideringsilkproteinisacellculture-basednaturalscaffold,weexploret

5、heimpactofmicro-andnano-patternedsilkproteinmembranesoncellbehaviorandfunction.Thispaperprovidesanewideafortissueengineeringresearchandhasimportantpracticalsignificance.Themaincontentsofthispaperareasfollows:1.Thepreparationofavarietyofsilkfibroinmembranewithmicro-nanosurfacetopol

6、ogy:Weusedelectronbeamlithographytoacquireavarietyofmicro-andnano-patternedpoly(methylmethacrylate)templateandeventuallyobtainedthecorrespondingmicroandnanopatternedsilkproteinmembrane.Inaddition,multipleanalyticalmethodswereemployedincharacterizingtheobtainedmicro-andIII万方数据Abstr

7、actThePreparationandapplicationofMicro-andNanopatternedSilkFibroinFilmsnanopatternedsurface.Theresultsshowedthatwecangetalargeareapattern2(approximately16mm),smallsized(about300nm),avarietyofpatterns(squarewell,pillars,pillars&well,multi-sizelines),water-insolublesilkfibroinfilm.2

8、.Preparationofchemicalmodifiedmic