砷对大鼠肝脏膜转运蛋白MRP2影响的研究

《砷对大鼠肝脏膜转运蛋白MRP2影响的研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、山西医科大学·2003届硕士学位论文·THEToXlCEFFECTSoFARSENlCoNTHEEXPRESSl0N0FLIVERMEMBRANETRANSPoRTPRoTEIN(MultidrugResistance—AssociatedProtein2)INRATSAbstractobjectiveToobservearsenictoxiceffectsonrat’Sliverandinvestigatetheroleofcanalicularmultispecificorganicaniontransporter／multidrugresistance—asso

2、cimedprotein2(cMOAT／MRP2)inthebilesecretionofarsenic．MethodFirstly，acutetoxicitywasconductedbyoraladministrationusingHorn‘Smethodtogethalflethaldose(LDso)．Secondly．thirtyhealthyratsweredividedrandomlyintosixgroupsbasedontimeintervalindoseof20mg／kg：2w、4w、6Wandtheircounterpartsofcontrolgr

3、oups．Theywereadministeredeveryotherday．Arsenicinbloodandbileweredetectedbyatomicabsorptionspectroscopy(AAS)，andusingWesternblottingtheexpressionofMRP2inthemembraneofhepatocytewasdetermined．Bylightmicroscopyandeletricmicroscopy，themorphologicalchangeswereobserved．Thirdly，sixbiochemicalin

4、dexes：Alanineaminotransferase(ALT)，sulthydrylgroups(SH)．totalbilirubininserum，sialicacid(SA)，glutathione山西医科大学·2003届硕士学位论文·peroxidase(GSH—PX)，andmalonicacid(MDA)wereselectedtoshowthetoxicel"rectsonrats．ResultsTheLDsoofarseniteis43mg／kg．Totalarseniclevelsinbloodandbileweresignificantlyhi

5、gherthancontrolgroups(P<0．05)atallthreedifferenttimepoints．TheupexpressionofMRP2was36．6l％、32．36％、12．73％respectivelyrangingfrom2Wto6W(percentageofcontrols，P

6、naliculimicrovillibecameswellingandsparse．Atthesametime，theshapeofnuclearshowedirregularandsomeofmitochondrialcristaemissedorbrokenbyelectricmicroscopycomparedwithcontrolgroups．ALTinserumwasonlyaffectedat6wandmarkedlyraised．Totalbilirubininserumwasincreasedat4wand6wtimepoints．SHinserumw

7、asfoundalmostunresponsivetoarsenic．MDAinserumandGSH—PXinbloodwereincreased(P