慢性排斥反应移植肾组织TIAF1的表达及其意义

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1、慢性排斥反应移植肾组织TIAF-1的表达及其意义作者：周广臣何小舟巢志复【摘要】目的探讨移植肾慢性排斥反应（CR）时转化生长因子-β1诱导的抗凋亡因子-1（TIAF-1）的表达及其意义。方法采用SP免疫组织化学染色法对6例因肾细胞癌行肾切除的未受肿瘤侵犯的正常肾组织和8例供肾正常肾组织、16例急性排斥反应和28例慢性排斥反应移植肾组织中TIAF-1的表达进行观察，并对间质浸润细胞中CD3、CD20、CD68阳性细胞数进行分析。结果正常供肾组织未见TIAF-1表达。急性排斥反应移植肾间质TIAF-1阳性细胞数明显增加，肾

2、小管上皮细胞呈微弱的TIAF-1表达。在慢性排斥反应移植肾组织中，TIAF-1在间质浸润细胞中的表达呈弥漫性、强阳性,较多的肾小管上皮细胞呈高度染色。移植肾间质浸润的TIAF-1阳性细胞数与CD3和CD20阳性细胞数呈正相关。结论TIAF-1在慢性排斥反应移植肾组织中的表达可能与Th1细胞向Th2细胞的免疫偏离有关，移植肾肾小管上皮细胞的TIAF-1表达可能反映了某种保护性机制的诱导。【关键词】肾移植；慢性排斥反应；转化生长因子-β1诱导的抗凋亡因子-1Abstract:ObjectiveToinvestigateth

3、echaracteristicsofTGF-β1-inducedantiapoptoticfactor-1(TIAF-1)expressioninrenalallograftofchronicrejection(CR)andtostudythe9possiblepathogenicroleofTIAF-1inchronicallograftrejection.MethodsImmunohistochemistryofstreptavidin-perosidase(SP)methodwasappliedintheobse

4、rvationoftheexpressionofTIAF-1ineachgroup,includingtissuesobtainedfromtheunaffectedpartofnephrectomizedkidneyswithrenalcellcarcinoma(n=6)andcadavericdonorofnormalkidneys(n=8)asthecontrolgroup,tissueswithacuterejection(AR,n=16)andthosewithchronicrejection(CR,n=28

5、),andintheanalysiscombinedwiththenumberofpositivecellsofCD3,CD20andCD68infiltratedintheinterstitium.ResultsTheexpressionofTIAF-1wasnotdetectableinnormalkidney.TheexpressionofTIAF-1positivecellsintheinterstitiumofrenalallograftswithacuterejectionwasup-regulatedan

6、dTIAF-1wasweaklyexpressedintubularepithelialcells.Intissueswithchronicrenalallograftrejection,ahighanddiffusiveexpressionofTIAF-1wasfoundinthepositivecellsinfiltratedintheintersitiumoftherenalallografts,withmoretubularepithelialcellshighlystained,mostofwhichwere

7、CD3andCD20positivecells.ConclusionTheexpressionofTIAF-1inrenalallograftswithchronicrejectionmightbecorrelatedwiththeimmunedeviationofTh1toTh2cells.TheexpressionofTIAF-1intubularepithelialcellsofrenalallograftsmay9beinducedbyacertainprotectivemechanism.Keywords:r

8、enaltransplantation;chronicrejection;TGF-β1-inducedAnti-apoptoticFactor-1(TIAF-1)慢性排斥反应（chronicrejection,CR）是器官移植后期移植物失功能的主要原因，制约着移植受体长期生存率的提高，其确切的发病机制目前尚未阐明。研究表明，凋亡在