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1、JournalofEnvironmentandHealth,May2000,Vol.17,No.3环境与健康杂志2000年5月第17卷第3期·167·【技术与方法】文章编号:1001-5915(2000)03-0167-04两种方法测定化妆品防腐剂抑菌效果及评价11112符晓梅封幼玲诸元生陆跃良张建新(1.江苏省卫生防疫站,南京210009;2.昆山市卫生防疫站)摘要:[目的]检测化妆品防腐剂抑菌效果,并对方法进行评价。[方法]选择两种凯松(凯松1、凯松2)和一种布罗波尔化妆品防腐剂,分别以纸片法和防腐剂效力测试法
2、观察了不同浓度(0.04%、0.06%、0.08%、0.1%和0.2%)和不同时间(6h、24h、48h、7d和14d)防腐剂对大肠杆菌ATCC8099、金黄色葡萄球菌ATCC6538、白色念珠菌ATCC10231和黑曲霉ATCC16404的抑菌效果。[结果]凯松1、凯松2和布罗波尔抑菌有效浓度纸片法测定分别为0.1%、0.06%和0.2%,在所试浓度内只有布罗波尔对金黄色葡萄球菌、白色念珠菌和黑曲霉无抑菌效果,防腐剂效力测试法测得3种防腐剂对大肠杆菌的抑菌有效浓度为0.04%,0.04%和0.2%。[结论]与纸片
3、法比较,防腐剂效力测试法考虑了化妆品化学成分对防腐剂抑菌效果的影响,更适合测定化妆品防腐剂抑菌效果。关键词:化妆品;防腐剂;抑菌中图分类号:R168文献标识码:BAssessmentandApplicationofTwoMethodsDeterminingtheBacteriostaticEffectsofCosmeticAntiseptic.FUXiao-mei,FENGYou-ling,ZHUYuan-sheng,etal.JiangsuProvincialSanitationandAnti-epidemicS
4、tation,Nanjing,210009Abstract:[Objective]Todeterminethebacteriostaticeffectsofcosmeticantiseptic,andtoassessetheaccuracyoftheassayresults.[Methods]Thebacteriostaticeffectsofthreekindsofcosmeticantiseptic,ofwhichtwowerekathonandonewasBronopol,onobservedstandard
5、bacteria,suchasE.coliATCC8099,stephaureusATCC6538,CandidaalbicansATCC10231andaspergillusnigerATCC16404,treatedbydifferentantisepticconcentrationsof0.04%,0.06%,0.08%,0.1%and0.2%andwithdifferentexposureperiodsof6,24and48hours,and14days,weredeterminedwithdisc-pap
6、erassayandantisepticeffectivenessassay.[Results]Thebacteriostaticeffectiveconcentrationsofkathon1,kathon2andBronopoldectectedbydisc-paperassaywere0.1%,0.06%and0.2%respectively,invalidantisepticeffectivenessdectectedbydisc-paperassayasonlyobservedinstephaureus,
7、condidaalbicansandaspergillusnigerexposedtoBronopol.Thebacteriostaticeffectiveconcentrationsdetectedbyantisepticeffectivenessassaywere0.04%,0.04%and0.2%forE.colitreatedbythreekindsofobservedantisepticmentionedabove.[Conclusion]Comparedwithdisc-paperassay,thean
8、tisepticeffectivenessasscey,reflectingthesyntheticeffectsofchemicalcompositionsofcosmeticontheantisepticeffectiveness,wassuitablefordeterminingthebacteriostaticeffectsofcosmeticant
