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1、上海交通大学硕士学位论文量子点的在免疫分析中的应用姓名：邵君申请学位级别：硕士专业：化学工程指导教师：高峰20070101上海交通大学硕士论文测物量存在相关性，据此建立了量子点-纳米金免疫荧光淬灭分析法定量检测乙肝表面抗原，检测极限达0.928ng/mL。以上两种方法只需检测反应体系中量子点荧光强度的变化就可检测靶物质的浓度，不需要分离抗原抗体的复合物，可直接检测，该方法简单方便，灵敏度高，在免疫分析和疾病诊断中有着广阔的应用前景。关键词：量子点，蛋白质，纳米金粒子，免疫分析，荧光淬灭7上海交通大学硕士论文APPLICATIONSOFQUANTUMDOTSI
2、NIMMUNOASSAYSABSTRACTQuantumdots(QDs),asaclassofnanomaterialswithmanyuniqueproperties,havebeenexploredpotentialapplicationsinbiomoleculardetection.Incompassionwithorganicdyes,QDsoffersuchadvantagesasbetterfluorescencestabilities,narrowemissionbandwidthsandbroadabsorptionspectra.Asa
3、markerappliedinimmunediagnoseandimmunoassay,itisatrendthatQDsareinsteadofthreetraditionallabeledtechnologies.ItisimportanttoresearchthequantificationalimmunedetectionbyQDs.HereinnovelimmunoassayswereestablishedbyQDsfortheirfluorescenceproperties.InfluencesofpH,buffers,temperature,s
4、avetime,aminoacidsandproteinsonfluorescenceofQDswerestudied.QDslinkedwithproteinsbyEDC.CompetitiveinhibitionELISAmethodofmagneticparticles-SAandCdTe-SAtoBiotin-HRPanddotimmuno-percolationwereusedtodetectthebiologicactivityofproteinsafterlabeling.TheresultsprovedthattheCdTe-ptoteinc
5、ompositecouldbeappliedinimmunoassaysasithadgooddispersivityandgoodbioactivity.UtilizingthefluorescencepropertiesofQDs,twonovelimmunoassayswereestablished,whichweretheaggregation-basedimmunoassayandfluorescencequenchingimmunoassay.ThesetwomethodswereusedtodetectImmunoglobulin(IgG)an
6、dHBsAg.Theaggregation-basedimmunoassayfordetectingMouseIgGusingQDshasbeendevelopedbyusingtwomatchedantibodies.TheGoatanti-MouseIgG(GMIgG)labelledquantumdotswasmixedwithMIgG.AftertheimmunoreactionbetweenGMIgGandMIgG,thequantumdotsaggregatedtoformanetwork,whichcouldquenchthefluoresce
7、nceofthemselvesinthereactionsystem.ThemoreMIgGwas,thebiggerthenetworkwas,andthefluorescenceintensityofquantumdotsdecreasedlinearly.TheconcentrationofMIgGcouldbedetectedbythemeasurementofQDsfluorescenceintensityat567nm,andthelimitdetectingconcentrationwas1.3nmol/L.QDs-GNPsfluorescen
8、cequenchingimmunoassaywasd
