(医学保健)组织及动物整体水平转基因获得的转基因动物

《(医学保健)组织及动物整体水平转基因获得的转基因动物》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。

1、精品PPT课件浏览免费下载后可以编辑修改。http://www.docin.com/jn-lxhhttp://www.doc88.com/lianer2012http://www.baidu.com/http://www.google.com/http://www.sogou.com/医学精品课件最权威的课件资料，免费在线阅读分享，下载后可以编辑修改。BasicstrategyofgenefunctionanalysisSection1.transgenicmodel组织及动物整体水平转基因获得的转基因动物Transgenicmodelsobtaine

2、donTissueandwholeanimal细胞水平转基因获得的基因转染细胞模型Transgeniccellmodelsobtainedoncellularlevel1、transgenicanimalsTransgenictechnology:Exogenousgeneisintroducedintofertilizedeggcellorembryonicstemcellandrecombinedrandomlyonchromosome,resultinginthatexogenousgeneisinsertedintogenomeofrecipi

3、entcellandpassontothenextgeneration.Transgenicanimal：exogenousgeneisintroducedandintegratedintogenomebymanipulativemethodsandobtaintheanimalharboredthegene.feature:tooperateonmolecularorcellularlevel,toexpressontissueorwholeanimallevel.BasicprocesspreparingtransgenicanimalThevec

4、torscarryingthedesiredgeneareinductedintothefertilizedeggsorembryoniccellswithmicroinjectionorothermethods.Thefertilizedeggorembryoniccellofcontainingadesiredgeneisimbeddedintooviducttodeveloptransgenicanimal,Therelationbetweenthegeneandthephenotypeofanimalisanalyzedtounderstand

5、thefunctionofthedesiredgene.Thetechniqueofgeneintroduction:physic,chemicalandbiologicmethods.1)microinjection2)embryonicstemcells(ES)3)retroviralinfection4)SpermatozoabearermicroinjectionSeparatingandcultureofembryonicstemcellBlastodermicvesicleSeparatingEndotheciumcellCellcultu

6、reEmbryonicstemcellbasicprocesspreparingtransgenicanimalApplicationoftransgenicanimal研究基因表达的组织或阶段特异性通过研究转入外源基因后的新表型，可以发现基因的新功能外源基因的随机插入，可能导致某内源基因突变，分析这些突变表型，可发现新基因用于只在胚胎期才表达的基因的结构和功能的研究建立研究外源基因表达、调控的动物模型研究遗传性疾病建立人类疾病的动物模型，为人类的基因治疗提供依据动物新品种的培育基因产品的制备用于对免疫机制、免疫相关疾病的研究及建立免疫性疾病动物模型2

7、、cellmodelsofgenetransfectiontocloneadesiredgeneintoeukaryoticexpressionvector→totransfectcell→toobtainthecellmodelsofgeneover-expression.basicprocessSeparationandpurificationofdesiredgeneToclonethegenetoexpressivevectorTotransfecttherecombinantvectorintocellSeparationandpurific

8、ationofdesiredgeneToclonethegenetoexpressivevec