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ID:35169784
大小:2.41 MB
页数:57页
时间:2019-03-20
《刚地弓形虫rh株rop10基因真核表达载体的构建及其免疫原性分析》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、授予单位代码10089学号或申请号12377Y於逭钭大5HebeiMedicalUniversity硕士学位论文在职科学学位刚地弓形虫RH株ROPIO基因真核表达载体的构建及其免疫原性分析学位申请人:姜文静导师:张进顺教授专业:病原生物学二级学院:河北北方学院2015年3月河北医科大学学位论文使用授权及知识产权归属承诺本学位论文在导师(或指导小组)的指导下,由本人独立完成。本学位论文研究所获得的研究成果,其知识产权归河北医科大学所有。河北医科大学有权对本学位论文进行交流、公开和使用。凡发表与学位论文主要内容相关的论文,第一署名为单位河北医科大学,试验材料、原始数据、
2、申报的专利等知识产权均归河北医科大学所有。否则,承担相应法律责任。I刀0:导师签章:f级学院领导盖章:yyrfr牟T月^日河北医科大学研究生学位论文独创性声明本论文是在导师指导下进行的研究工作及取得的研究成果,除了文中特别加以标注和致谢等内容外,文中不包含其他人已经发表或撰写的研究成果,指导教师对此进行了审定。本论文由本人独立撰写,文责自负。研究生签名g对目录中文摘要·············································································1英文摘要····················
3、·························································3英文缩写·············································································6研究论文刚地弓形虫RH株ROP10基因真核表达载体的构建及其免疫原性分析前言·············································································8材料与方法··························
4、··········································9结果·············································································23附图·············································································26附表·············································································33讨
5、论·············································································34结论·············································································36参考文献·······································································37综述弓形虫疫苗的研究进展·····································
6、·············40致谢···················································································52个人简历·············································································53中文摘要刚地弓形虫RH株ROP10基因真核表达载体的构建及其免疫原性分析摘要目的:弓形虫(Toxoplasmagondii)是世界性分布的寄生虫。该虫是重要的机会性致病原虫,寄生于人体及动物的有核细胞内,
7、可感染人体和全球200多种动物,引起弓形虫病。据估计全世界30%的人群感染本虫。免疫功能低下和新生儿是弓形虫病的最大受害者,可引起严重的后果,直接影响到人类的健康和社会的发展,同时对食品公共卫生安全和畜牧业也造成了一定程度的隐患和危害,而目前尚没有理想的防治弓形虫病的药物,所以研制安全有效的疫苗预防该病尤为重要。研究表明弓形虫棒状体蛋白由棒状体分泌,对弓形虫侵入宿主细胞并对其发挥毒力方面均起着重要的作用,在疫苗研制方面具有很大的潜力。本研究通过分子生物学方法构建了重组质粒pVAX1-ROP10,并将其肌肉注射免疫BALB/c小鼠,为进一步研究弓形虫R
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