Gateway &ampamp;reg; Technology with Clonase&ampamp;#8482; I.pdf

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1、®GatewayTechnologywith™ClonaseIIAuniversaltechnologytocloneDNAsequencesforfunctionalanalysisandexpressioninmultiplesystemsCatalognos.12535-029and12535-037Revisiondate:07November2010Manualpartno.25-0749MAN0000470ALimitedUseLabelLicensecoversthisproduct(seePurchaserNotification).Byuseofthispro

2、duct,youacceptthetermsandconditionsoftheLimitedUseLabelLicense.CorporateHeadquartersInvitrogenCorporation1600FaradayAvenueCarlsbad,CA92008T:17606037200UserManualF:17606026500E:tech.service@invitrogen.comForcountry-specificcontactinformationvisitourwebsiteatwww.invitrogen.comiiTableofContents

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4、oducts...................................................................................................................................................ixIntroduction.......................................................................................................................1Overv

5、iew.....................................................................................................................................................................1TheGateway®Technology......................................................................................................

6、..................................3Gateway®BPandLRRecombinationReactions....................................................................................................5FeaturesoftheGateway®Vectors...........................................................................................

7、.................................8Gateway®Nomenclature...........................................................................................................................................9Methods...............................................