pCR&ampamp;reg;8GWTOPO&ampamp;reg; TA Cloning&ampamp;reg; Kit.pdf

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1、®®®pCR8/GW/TOPOTACloningKit®Five-minute,TOPOCloningofTaqpolymerase-amplifiedPCRproductsintoanentryvectorforthe®GatewaySystemCatalognos.K2500-20,K2520-20,andK2520-02VersionE10April200625-0706CorporateHeadquartersInvitrogenCorporation1600FaradayAvenueCarlsba

2、d,CA92008T:17606037200UserManualF:17606026500E:tech.service@invitrogen.comForcountry-specificcontactinformationvisitourwebsiteatwww.invitrogen.comiiTableofContentsTableofContents...............................................................................

3、........................................................................iiiTOPO®CloningProcedureforExperiencedUsers................................................................................................vKitContentsandStorage.......................

4、..................................................................................................................viiAccessoryProducts..........................................................................................................................

5、..........................xIntroduction.......................................................................................................................1Overview.........................................................................................

6、.............................................................................1ExperimentalOutline.................................................................................................................................................4Methods......

7、........................................................................................................................5DesigningPCRPrimers....................................................................................................................

8、.........................5ProducingPCRProducts...........................................................................................................................................7SettingUptheTOPO®Cloni