multi-environment qtl analysis of grain morphology traits and fine mapping of a kernel-width qtl in zheng58 × sk maize population

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1、、＇ｍＩ＇式心Ｖ怒？Ｊ，ｒ４，＃＋ＩＩＡ＃■■ｌｉｉｊＨＨＨＵＡＺＨＯＮＧＩＶＥＲＳＩＴＹ：ＡＧＲＩＣＵＬＴＵＲＡＬＵＮ＾＾；Ｉ博±学位论文ｉ化ＤＤＩＳＳＥＲＴＡＴＩＯＮ■Ｍ＿Ｍ＿Ａ＿Ｍ＿ＹＴＲＡＩＴＳ－ＡＮＤＦＩＮＥＭＡＰＰＩＮＧＯＦＡＫＥＲＮＥＬＷＩＤＴＨＴＬＩＮＱＵＨＺＨＥＮＧ５８ＸＳＫＭＡＩＺＥＰＯＰＵＬＡＴＩＯＮｉｌ■Ｉ妍究生：穆罕默徳謝里夫拉伊汉ＣＡＮＤＩＤＡＴＥ：ＭＯＨＡＭＭＡＤＳＨＡＲＩＦＲＡＩＨＡＮＢ导师；严建兵教授ｍＳＵＰＥＲＶＩＳＯＲ：ＰＲＯＦＥ

2、ＳＳＯＲＪＩＡＮＢＩＮＧＹＡＮｉｌｙ专业：遗传学ＵＭＡＪＯＲ：ＧＥＮＥＴＩＣＳＨ研究方向：玉米遗传学ＵＨＦＩＥＬＤ：ＭＡＩＺＥＧＥＮＥＴＩＣＳＵＬ中国武汉ＷＵＨＡＮＣＨＩＮＡ国■！，二〇一六年六月ｎｍＨＨＪＵＮＥ，２０１６ｊ分类号密级华中农业大学博士学位论文MULTI-ENVIRONMENTQTLANALYSISOFGRAINMORPHOLOGYFINEMAPPINGANDCLONINGOFAMAJORQTLAFFECTINGTRAITSANDFINEMAPPINGOFAKERNELKERNELSIZEINMAIZ

3、E(Zeamays-WIDTHQTLINL.)ZHENG58×SKMAIZEPOPULATION研究生：MOHAMMADSHARIFRAIHAN学号：2012304060003指导教师：PROFESSORJIANBINGYAN指导小组：YongzhongXingLinglingChenZuxinZhangMingqiuDaiJianbingYan专业：GENETICS研究方向：MAIZEGENETICS获得学位名称：博士获得学位时间：2016年6月华中农业大学生命科学技术学院二○一六年六月June2016DedicatedtoMybelovedwifeDr.FougeaKadirAnd

4、OurhybridMohammadFaiyajRaihanMulti-environmentQTLanalysisofgrainmorphologytraitsandfinemappingofakernel-widthQTLinZheng58×SKmaizepopulationTableofContentsPageNo.摘要………………………………………………………...iAbstract………………………………………………………..iiListofAbbreviation…………………………………………...iiiListofTables………………………………………………….ivL

5、istofFigures…………………………………………………vListofAppendices……………………………………………viCHAPTERIINTRODUCTION…………………………………………….11.1BreedingforMaizeImprovement…………………………11.1.1Maize(ZeamaysL.)……………………………….11.1.2Geneticsofkerneldevelopment…………………...21.1.3Maizekernelrelatedtraitsandyield……………….31.2PositionalCloningAnalys

6、istoDissectComplexTrait…...31.2.1Mappingpopulation………………………………..41.2.2Choiceofmappingpopulation……………………..41.2.3Constructionoflinkagemap……………………….51.2.4QTLanalysisandmapping………………………...61.2.5Finemapping……………………………………….71.2.6Positionalcloning…………………………………..71.3QTLAnalysisofMaizeGrainMorphologyTrai

7、tsBasedon8RecombinantInbredLine(RIL)Population1.3.1QTLsformaizekerneltraits………………………..81.3.2QTLanalysisinRILpopulation…………………….101.4FineMappingandMap-basedCloningUsing10HeterogeneousInbredFamily(HIF)1.4.1Finemappingofgrainwi