丙型肝炎病毒感染者mir-181a对cd4+t细胞衰老的调节作用及信号传导

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1、分类号R512.63第四军医大学UDC616.9TheFourthMilitaryMedicalUniversity密级公开博士学位论文丙型肝炎病毒感染者miR-181a对CD4+T细胞衰老的调节作用及信号传导周云培养类别全日制学位类型学术学位一级学科(专业类)临床医学二级学科(专业)内科学（传染病）研究方向丙型肝炎病毒免疫机制指导教师贾战生教授（主任医师）培养单位唐都医院传染科二O一五年五月第四军医大学博士学位论文目录缩略语表···········································································

2、····················1中文摘要·······························································································5英文摘要·······························································································7前言····································································

3、································10文献回顾······························································································11正文····································································································24实验一HCV感染者CD4+T细胞miR-181a抑制DUSP6表达····················

4、··241材料····························································································241.1外周血来源·············································································241.2试剂·······················································································251.3仪器和耗材·

5、············································································262方法····························································································272.1CD4+T细胞分离和培养·····························································272.2miScriptmiRNAarray·················

6、··············································272.3共培养CD4+T细胞和HCV-或HCV+Huh7肝细胞··························282.4流式细胞术检测·······································································292.5实时定量RT-PCR检测miR-181a和DUSP6····································292.6纯化CD4+T转化miR-181aprecursors·····

7、······································312.7阻断DUSP6············································································322.8统计学处理·············································································323结果····································