th17细胞及其相关细胞因子与慢性hbv感染疾病进展的相关研究

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1、分类号R575UDC616.3密级公开Th17细胞及其相关细胞因子与慢性HBV感染疾病进展的相关研究蔡云培养类别全日制学位类型专业学位一级学科(专业类)临床医学二级学科(专业)内科学研究方向慢性肝病的综合防治指导教师韩英教授（主任医师）培养单位西京医院消化科二O一五年五月第四军医大学硕士学位论文目录缩略语表····························································································1中文摘要·································

2、···························································4ABSTRACT·························································································7前言···························································································11文献回顾···························

3、································································13正文···························································································26第一部分Th17细胞及相关细胞因子在慢性HBV感染病程中变化的研究............261材料.........................................................................

4、............................................261.1研究对象··············································································261.2生化试剂与材料·····································································271.3主要仪器·······························································

5、···············281.4主要试剂配制·········································································292方法.....................................................................................................................292.1血浆采集与保存···················································

6、··················292.2ELISA检测IL-17、IL-21、IL-22、IL-23浓度································302.3外周血单个核细胞采集及保存···················································312.4细胞复苏及培养、刺激····························································312.5流式细胞术检测Th17、Th22百分比·····························

7、··············322.6统计学处理···········································································323结果.....................................................................................................................323.1研究对象一般临床资料················································

8、············