rna结合蛋白hur对乳腺癌细胞中pdgfc的转录后调控机制研究

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1、分类号R737.9UDC616-006密级公开RNA结合蛋白HuR对乳腺癌细胞中PDGFC的转录后调控机制研究罗年安培养类别全日制学位类型学术学位一级学科(专业类)临床医学二级学科(专业)外科学（普外）研究方向乳腺癌的生物学治疗指导教师董瑞副教授（副主任医师）培养单位唐都医院普通外科二O一五年五月第四军医大学硕士学位论文目录缩略语表···············································································································

2、·············1中文摘要····························································································································3英文摘要····························································································································6前言····

3、························································································································9文献回顾··························································································································11正文···························

4、·······························································································19实验一PDGFC在乳腺癌组织和细胞系中的表达···················································191材料、试剂和仪器·······························································································19

5、1.1实验材料········································································································191.2主要试剂和仪器····························································································202方法··························································

6、·····························································212.1PDGFC免疫组织化学检测············································································212.2乳腺癌细胞培养····························································································222.3蛋白印迹法（Westernb

7、lot）········································································222.4siRNA（PDGFC）转染·················································································242.5MTT实验····························································································

8、···········242.6侵袭实验··································