sox2在不同发育阶段sd大鼠穹窿下器细胞的表达

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1、授予单位代码10089学号或申请号13327HebeiMedicalUniversity硕士学位论文在职科学学位Sox2在不同发育阶段SD大鼠穹窿下器细胞的表达学位申请人：王榕导师：马常升教授专业：人体解剖与组织胚胎学二级学院：基础医学院2015年3月河北医科大学学位论文使用授权及知识产权归属承诺本学位论文在导师(或指导小组〉的指导下，由本人独立完成。本学位论文研究所获得的研究成果,其知识产权归河北医科大学所有。河北医科大学有权对本学位论文进行交流、公开和使用。凡发表与学位论文主要内容相关的论文,第一署名为单位河北医科大学，试验材

2、料、原始数据、申报的专利等知识产权均归河北医科大学所有。否则，承担相应法律责任。研究生签名:¥导师签章j河北医科大学研究生学位论文独创性声明本论文是在导师指导下进行的研究工作及取得的研究成果,除了文中特别加以标注和致谢等内容外,文中不包含其他人已经发表或撰写的研究成果,指导教师对此进行了审定。本论文由本人独立撰写,文责自负。年_r月>曰目录中文摘要··············································································1英文摘要··········

3、····································································4英文缩写··············································································7研究论文Sox2在不同发育阶段SD大鼠穹窿下器细胞的表达前言··············································································8材料与方法··

4、···································································8结果·············································································12附图·············································································15讨论····································

5、·········································21结论·············································································23参考文献·······································································23综述神经干细胞培养鉴定及移植应用展望································25致谢·············

6、······································································34个人简历·············································································35中文摘要Sox2在不同发育阶段SD大鼠穹窿下器细胞的表达摘要目的：观察出生后不同发育阶段大鼠穹窿下器（subfornicalorgan,SFO）的Sox2阳性细胞的形态及分布特征，观察其表达情况。方法：雄性健康SD大鼠,分为幼

7、年组和成年组，每组各24只，清洁级，实验动物不作任何处理。1标本制备常规动物标本制备：将两组实验动物用水合氯醛（0.3ml/100g）麻醉后，自主动脉灌注4%多聚甲醛磷酸盐缓冲液（0.1mol/L，PH7.2）进行前固定，立即取脑，进行后固定。将组织标本经脱水，透明，包埋，选取含穹窿下器部分，石蜡切片机冠状面切片，厚度约为5μm，制成石蜡切片。对实验动物的处置符合动物伦理学要求。2染色常规免疫组织化学染色：将上述石蜡切片经Sox2染色，使用中性树胶封片后，显微镜观察、照相。3统计学处理选取相同SFO的横断面，使用多媒体真彩病理图文

8、分析系统，在20倍镜下，通过计数SFO内细胞总数和Sox2阳性细胞数，计算出阳性细胞率，计量资料均采用x±s表示。结果：1不同发育阶段大鼠Sox2阳性细胞在SFO的分布。幼年组大鼠的SFO，腹侧部的室管膜Sox2阳性细胞排列整齐而密集，中央部的室管