转录因子runx3对前列腺癌细胞恶性表型的影响

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1、分类号R69UDCR697+.3密级公开转录因子RUNX3对前列腺癌细胞恶性表型的影响陈勇培养类别全日制学位类型学术学位一级学科(专业类)临床医学二级学科(专业)外科学（泌外）研究方向前列腺癌基因治疗指导教师王禾教授（主任医师）培养单位唐都医院泌尿外科二O一五年五月独创性声明彔承学校严逆的学风与优良的科学边埝，本人声明所里交的论文是ftr人在导陌指导下进H的研究工作及取得的研究成果。尽ft瓶知，除了文中特别M以疼注和致谢的地)1外，坨文中不0含其他人已经发表或搆写过的研究成果，不包含本人成他人已申请学位或料他丨丨I

2、途使用il的成梁。与我一同工作的同志对本研究所做的任I"丨奇献均已在论文中作了明辑的说明并表示了致谢。申请学位论文与资料若有不实之处，本人承担一W相关责任。坨文作者签名：日期：保护知识产权声明本人完全了解第0军g大学有矢保护知识产权的〖I定，I：研究生在校攻玟学位期间论文工作的知识产权单位为第0军医大学。本人保姐毕业读校后，发表论文等使用论文工作成果时第？！名申位仍然H第0军医大学。学校可以公布论文的全部或部计内容丨含电子ffi,保密内容除外），可以采用彤印，缩印或其他+K保存论文；学校ft权允并论文校查阅和丨SP

3、il,并任校园网上S供论文内容的31览和下IUR务。问g学校将论文加入《中IS优秀博砷士学位论文全文数据库》和编入《中0知识资®&序》等，并可浏览和下钱，问时享受《中国优秀W碘士学位论文全文数拥障出ffi草fl>报定的相关权fi�论文作者签名：导师签8:R期：第四军医大学博士学位论文目录缩略语表································································································1中文摘要···········

4、·····················································································3英文摘要································································································5前言·······································································

5、·························8文献回顾·······························································································10正文·······························································································24实验一RUNX3-flag基因重组腺病毒载体的构建及其在前列腺癌细胞

6、中的表达和定位分析·················································································251前言····························································································252材料和仪器·································································

7、··················263方法····························································································274结果····························································································355讨论···········································

8、·················································38实验二转录因子RUNX3对PC-3前列腺癌细胞恶性表型的影响··················411前言············································································