早产儿喂养不耐受与肠道菌群相关性研究

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1、分类号R722.6第四军医大学UDC616TheFourthMilitaryMedicalUniversity密级公开硕士学位论文早产儿喂养不耐受与肠道菌群相关性研究张良娟培养类别全日制学位类型专业学位一级学科(专业类)临床医学二级学科(专业)儿科学研究方向小儿消化系统疾病指导教师王宝西教授（主任医师）培养单位第四军医大学唐都医院儿科二O一五年五月第四军医大学硕士学位论文第四军医大学硕士学位论文目录缩略词表.................................................................

2、.............................................................1中文摘要..............................................................................................................................4英文摘要.........................................................................

3、.....................................................6前言..............................................................................................................................9文献回顾...................................................................................

4、.........................................11正文..........................................................................................................................22第一部分应用PCR-DGGE研究喂养不耐受早产儿肠道微生物群落多样性.......220引言.......................................................

5、..................................................................221材料.........................................................................................................................231.1实验对象.....................................................................

6、......................................231.2粪便标本的收集，处理...................................................................................231.3主要试剂和材料...............................................................................................231.4主要仪器..................

7、.........................................................................................241.5主要溶液及试剂配制.......................................................................................252方法...........................................................................

8、..............................................272.1粪便基因组DNA提取.................................................