yap与p-yap在肝细胞癌中的表达与其临床生物学行为关系的研究

《yap与p-yap在肝细胞癌中的表达与其临床生物学行为关系的研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、授予单位代码10089学号或屮请号13326HebeiMedicalUniversity硕士学位论文在职科学学位YAP与P-YAP在肝细胞癌中的表达与其临床生物学行为关系的研究学位申请人：刘远光导师：李勇教授檀碧波副教授专业：外科学2015年3月河北医科大学学位论文使用授权及知识产权归属承诺本学位论文在导师（或指导小组）的指导下，由本人独立完成。本学位论文研究所获得的研究成果，其知识产权归河北医科大学所有。河北医科大学有权对本学位论文进行交流、公幵和使用。凡发表与学位论文主要内容相关的论文，第一署名为单位河北医科大学，试验材料、原始数据、申报的专利等知识产权均归河北医科大学所有。否

2、则，承担相应法律责任。河北医科大学研究生学位论文独创性声明本论文是在导师指导下进行的研究工作及取得的研究成果，除了文中特别加以标注和致谢等内容外，文中不包含其他人己经发表或撰写的研究成果，指导教师对此进行了审定。本论文由本人独立撰写，文责自负。目录中文摘要··············································································1英文摘要··············································································4英文缩写

3、··············································································7研究论文YAP与P-YAP在肝细胞肝癌中的表达与其临床生物学行为关系的研究前言··············································································8材料与方法·····································································9结果····················

4、·························································18附图·············································································21附表·············································································27讨论·······································································

5、······30结论·············································································33参考文献·······································································34综述YAP与恶性肿瘤的研究现状与进展····································37致谢······································································

6、·············46个人简历·············································································48中文摘要YAP与P-YAP在肝细胞癌中的表达与其临床生物学行为关系的研究摘要目的：肝细胞癌（HCC,hepatocellularcarcinoma）是肝脏原发肿瘤中最常见的类型，也是我国乃至全世界.最常见的恶性肿瘤疾病之一。通常HCC被发现时患者已进入晚期，常规化疗对其作用不明显，致使生存率较其它癌症显著降低。因此，加强对HCC的发病机制研究，寻找更多关于肝癌基因及抑癌基因的表达并明确其与

7、HCC的关系已成为当前研究的重要方向。Hippo通路在促进细胞凋亡，限制细胞增殖，调控器官分化方面起着重要的作用。YAP(Yes-associatedprotein)即Yes相关蛋白，作为该通路下游的效应因子，多以磷酸化形式（P-YAP）参与细胞信号转导以调控细胞凋亡与增殖的平衡。在病理状态下，被阻断或失活的Hippo通路对YAP磷酸化作用失效致YAP自身活性增强使细胞增生和凋亡间平衡失调、组织器官过度生长及肿瘤发生。国内外相关研究表明，YAP作为新近发现的癌基因，参