mtdh、iκb、nf-κb p65在乳腺癌组织中的表达及意义

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1、授予单位代码10089学号或申请号13681HebeiMedicalUniversity硕士学位论文MTDH、IκB、NF-κBP65在乳腺癌组织中的表达及意义作者姓名：张硕稳导师：宋振川教授专业：外科学二级学院：第四医院2015年03月河北医科大学学位论文使用授权及知识产权归属承诺本学位论文在导师（或指导小组）的指导下，由本人独立完成。本学位论文研究所获得的研究成果，其知识产权归河北医科大学所有。河北医科大学有权对本学位论文进行交流、公幵和使用。凡发表与学位论文主要内容相关的论文，第一署名为单位河北医科大学，试验材料、原始数据、申报的专利等知识产权均归河

2、北医科大学所有。否则，承担相应法律责任。河北医科大学研究生学位论文独创性声明本论文是在导师指导下进行的研究工作及取得的研究成果，除了文中特别加以标注和致谢等内容外，文中不包含其他人已经发表或撰写的研究成果，指导教师对此进行了审定。本论文由本人独立撰写，文责自负。>o-0"年A月/日目录中文摘要·············································································1英文摘要····················································

3、·························3研究论文MTDH、IκB、NF-κBP65在乳腺癌组织中的表达及意义前言·············································································6材料与方法····································································7结果·································································

4、············13附图·············································································15附表·············································································21讨论·············································································25结论······················

5、·······················································28参考文献·······································································29综述MTDH与肿瘤的研究进展···············································34致谢··················································································

6、·44个人简历·············································································45中文摘要MTDH、IκB、NF-κBP65在乳腺癌组织中的表达及意义摘要目的：乳腺癌（breastcancer）已成为当今女性最常见的恶性肿瘤,近年来其发病率和死亡率均呈现上升趋势。乳腺癌好发于45-55岁的女性，然而随着人们生存环境、生活方式以及饮食习惯的改变，发病年龄渐渐趋于年轻化。乳腺癌的形成原因复杂多样，它是一个多因素、多突变积累的结果。关于乳腺癌的发病机制存在很多学说。近几年来

7、，对乳腺癌分子生物学层面的研究已成为此领域研究的热点。MTDH又称为AEG-1、LYRIC，该癌基因位于人类8号染色体上（8q22），在多种肿瘤细胞中高表达，并与肿瘤细胞的增殖、侵袭、转移及耐药密切相关。IκB是核因子κB（NF-κB）的抑制蛋白。NF-κBP65是NF-κB的一个亚单位，它的高表达与肿瘤细胞的侵袭、转移密切相关。在细胞受到刺激时，三者通过共同作用诱导相关基因转录，促进肿瘤的发生发展。本实验通过实时荧光定量PCR及免疫组织化学法分别检测MTDH、IκB、NF-κBP65三者在乳腺癌组织及癌旁正常组织（距离癌灶>5cm）中mRNA及蛋白质含量

8、，并结合患者临床资料及病理学结果，进一步探讨三者在乳腺癌的发生、发