dna免疫研制艰难梭菌外毒素a和b单克隆抗体及抗体基因序列分析

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1、分类号：单位代码：10312密级：学号：20122087博士学位论文题目：DNA免疫研制艰难梭菌外毒素A和B单克隆抗体及抗体基因序列分析研究生：张春华指导教师：卢山学科专业：内科学（感染病学）学院名称：南京医科大学第一临床医学院完成时间：二〇一五年五月南京医科大学学位论文原创性声明本人郑重声明：所呈交的论文是本人在导师的指导下，独立进行研巧工作所Ｌ，本论文不包含任何其他个人取得的成果。除了文中特别加乂标挂引用的内容外或集体已经发表或撰写的成果作品。对本文的研巧做出重耍贡献的个人和集体，。均己在文中。本

2、人完全意识到本声明的法律后果由本人承担｜＾明确方式标明ｉ省＾吩作者签名：與辞日期：ｉＴ年＾月日导师签名：曰期＾如＾５不ｔ学位论文版权使用授权书、意学校保本学位论文作者完全了解学校有关保留使用学位论文的规定，同留并向国家有关部口或机构送交论文的复印件和电子版，允许论文被查阅和借阅。本人授权南京医科大学可将本学位论文的全部或部分内容编入有关数据库进行检索レ、缩印或扫描等复制手段保存和汇编本学位论文。，可ッ采用影印＂＂本学位论文属于（请务必在Ｗ下相应方框内打Ｖ）：１、保密□年解密后适用本授

3、权书。，在＿２、不保密Ｉ］。；：Ｔ年作者签名：蛛黄＾曰期州店月抑．副巧签名：ｃＰ刖扬冷月胃子ｊ南京医科大学博士学位论文目录英文缩略词······················································································3中文摘要·························································································5英文摘要····

4、·····················································································9前言·······························································································13正文1．艰难梭菌外毒素A单克隆抗体的研制及抗体特性研究1.1引言················································

5、································291.2材料和方法·······································································301.3结果················································································521.4讨论··························································

6、······················631.5参考文献··········································································662．艰难梭菌外毒素B单克隆抗体的研制及抗体特性研究2.1引言················································································712.2材料和方法······································

7、·································732.3结果················································································892.4讨论················································································992.5参考文献·················································

8、·······················1023．DNA免疫制备的针对多种抗原的单克隆抗体Ig基因克隆及抗体序列分析3.1引言··············································································1