人参种质资源遗传多样性分析及分子标记开发研究

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1、AbstractGinseng(PanaxginsengC.A.Mey.)hastheuniquebiologicalcharacteristicsthatlongcycleofgrowth,thenumericalofreproductioncoefficientissmall.Forthisreason,Ginsengbreedingworktohavecausedgreatdifficult.TherehavesofarbeenfoundthatmorethantenvariationtypesinGinsengspecies.Exceptthe

2、geneticcharacteristicswhichclassificationaccordingtothemorphologicaloftherootmorphologicalthatneedtobefurtherstudied,othertypesnotonlyhaveshownthegeneticcharacteristicsthereare,Butalsohastheirownvalue;andinthestudyofthepracticewhichhasprovenedthesetraitscanbeinheritedstability.O

3、ntheonehand,thisstudystartinGermplasmResourceswhichthesourceofbreedingwork,analysistheGeneticdiversityandphylogeneticrelationshipbetweenthedifferenttypesofGinsengcultivations.AnalysistheGeneticdiversityamongindividualswhichinthesameGinsengtypegroups.(GurplestemsGinsengasanexampl

4、e)Inordertoprovideatheoreticalbasisforthenewvarietiesofginsengbreedingwork.Ontheotherhand,InordertoimproveandspeeduptheworkofbreedingnewvarietiesofGinseng,usingRAPDmethod,developedtoexplorethemolecularmarkersbetweentheGreenandpurplestemsthesetwokindsofdifferenttypesofGinseng.And

5、analysistotheDNAsequence,transformitqintoSCARmaker.Thereportthatcontentsoftheexperimentareasfollows：1OptimizedthereactionsystemoftheRAPDanalysisofGinsenganddetermineaeconomic,efficientandreliableresponsesystem.Andapplicationtotheafterexperiment.2Analysisofgeneticdiversitytotheto

6、talof23samplesofpurplestemGinsengfromthesameculturegroupbyRAPDmethod.Identifiedthegeneticdifferencesamongthesamegroup.Andmappedthegeneticrelationshiptreemapofthesamplesandthesimilaritycoefficienttable.AnalyzedgeneticrelationshipofthesamegroupofstempurpleGinseng.Thedifferencerate

7、ofthesamplesis38.8%,Comparedwiththeotherplants,itmoreconservative.3Analysisofgeneticdiversityof4diffetenttypesofGinsengbyRAPDmethod.Identifiedthegeneticdifferencesinthe4differenttypesofGinseng.Andmappedthe万方数据geneticrelationshiptreemapsamplesandthesimilaritycoefficienttableofthe

8、4differenttypesofGinseng.Analysisofrelationship