中国假俭草种质资源遗传多样性研究

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1、Abs仃actAccordingtothedistributionstatusofthegermplasmresourcesofEremochloaophiuroidesfromdifferentprovinces：Hainan，Guangdong，Fujian，Zhejiang，Jiangsu，Anhui，Henan,Hubei，Jiangxi，Hunan，Chongqing，Sichuan,Yunnan，GuizhouandGuangxiprovinceinChina,investigationandcollectionWaSmadeandm

2、aterialswereanalyzedintwomajorlevels：morphologylevelandDNAlevel，totestthegeneticdiversityof60samplesofEremochloaophiuroidesgermplasmresourcesthatgatheredfromabovethirteenprovinces．IntegratedanalysisofmorphologylevelWaSmadeintwoparts：spaceenlargementofclonesandturfquality,thes

3、eanalysesprovidedbaSisforstudy,collection，newcultivatesbreedingandrationalutilizationofthegermplasmresourcesofEremochloaophiuroides．(1)．ResearchresultsofEremochloaophiuroidesmorphologicalmarkers：Amongthe1morphologicalmarkers，themaximumrangevariationWasintheleaflengthofvertica

4、lbranch，whichwasabout22．15％；theminimumrangevariationWasexistedthediameterofstolon，whichWaSonly7．82％．Of12characteristicsofstolonhorizontalenlargement,correlationanalysisshowedahighlysignificantlevel(P

5、ond—levelinternodesnumbers，first-levelnodeslength．Thestemintemodeslength,first-levelbranchintemodesnumbersandsecond-levelinternodeslengthshowedasignificantlevel．ThevariationrangeofeachmarkerWas0．51％,---3．28％，whichmeanstherichdiversityamongdifferentasexualpropagationcharacteri

6、zationmarkers．(2)．AssessmentofsixtygermplasmofEremochloaophiuroidesusingtheComprehensiveAssessmentSystemofTurfQualityproposedbyLiuJianxiu．Theresultsshowedthatamongdifferentvarietiesofturfgrasses，NO．54germplasmscoresthehighest，whileNO．48scoresthelowest，theleafmorphologyindexva

7、luerangesinthemiddlelevelintherestofgermplasm，theevaluationscoreproclaimedthatEremochloaophiuroidesissuitabletodifferentkindsofturf．(3)．GeneticdiversityofsixtygermplasmofEremochloaophiuroidesusingISSRmolecularmarkeranalysis．ThirteenhighlypolymorphismandresolutionpairsofISSRpr

8、imerswereusedtotheamplificationofgenomeDNAofmaterials，104piecesofrea