中国南瓜种质资源遗传多样性研究

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1、AbstractCucurbitaMoschataisoneofthethreemaincultivarsinCucurbita．Cucurbitamoschataisallimportanthorticulturalplantandhavemanyvariousfi'uitcolour．111estudyonthegeneticdiversityofCucurbitamoschatacanbenefittheconservation,classification,identification,gene

2、ticenhancementandeffectiveutilizationoftheCucurbitamoschatagermplasmrcsonrce$．Molecularmarker,whichCandetectthegeneticdiversityatDNAlevel，isapowerfultooltostudygeneticdiversity．RAPDmarkershavebeenpopularizedfortheirsimplicity,rapidityandnon-specialtyamon

3、gdifferentgenus．Inthisstudy,thegeneticdiversityin70CucurbitaMoschata．TheseaccessionsfromdifferentregionsofChinawereassessedusingagronomicu丑itsandRAPDmarkers，andthegeneticrelationshipamongtheaccessionsWaSrevealedbyclusteranalysis，principalcomponentsanalys

4、isandprincipalcoordinatesanalysisatvariouslevel．皿emainresultsw眦asfollows：1．Atotalof56agronomicuaitsweremeasured,31quantitativeand25qualitative，forassessinggeneticdiversityof70CucurbitaMoschatagermplasm．Theaveragecoefficientofvariationwas37．50％．Theyranged

5、丘啪6．60％inaspectratioofcotylendonto97．44％inlengthwisediameterpulpthicknessofripepumpkinbasedonthe31qualitativedescriptorsandfrom9．68％incircumferencecolourofseedto262．22％inbisexualflowerbasedonthe25quantitativedescriptors．TheaverageShannon'sInformationinde

6、xamongtheaccessionsWas1．39basedonthedataof56morphologicaldescriptors．Theyvariedfrom0．22to1．45basedonquantitativedescriptors．Theyvaried丘om1．43to2．49basedonqualitativedescriptors．AlltheresultsaboveconfirmedthattheShannon'sInformationindexbasedonquantitativ

7、edescriptorswashigherthenbasedonqualitativedescriptors．2．Exceptthe5germplasm,theclusteranalysisdividedthe65germplasminto2majorgroupsbasedonagronomictraits．OnewasroundpumpkinandanotherWaslongpumpkiILRoundpumpkinWasdividedintoexistingbumparoundfruitsurface

8、andsmoothfruitsurface．Theresultsofprincipalcomponentsanalysisaccordedwithclusteranalysis．3．21primerswerescreenedfrom100arbitraryprimel罄，andatotalof167DNAbandswereamplified。130(73．23％)ofwhichwerepolymorphic．n岵numberofbandsp