叶酸抑制血管平滑肌细胞去分化的分子机制研究

《叶酸抑制血管平滑肌细胞去分化的分子机制研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、分类号：R543.3单位代码：10343学号：151001140硕士学位论文论文题目:叶酸抑制血管平滑肌细胞去分化的分子机制研究研究生姓名：潘孙雷学科专业：内科学（心血管病）类型：学术型指导教师：郭航远二O一八年五月温州医科大学硕士学位论文论文题目:叶酸抑制血管平滑肌细胞去分化的分子机制研究答辩委员会主席:郑良荣浙江大学附属第一医院答辩委员会成员:季亢挺温州医科大学附属第二医院龚永生温州医科大学论文答辩日期:2018年5月27日1温州医科大学硕士学位论文目录论文„„„„„„„„„„„„„„„„„2缩略词表„„„„„„„„„„„„„„„„3中文摘要„„„„„„„„

2、„„„„„„„„5英文摘要„„„„„„„„„„„„„„„„7前言„„„„„„„„„„„„„„„„10材料与方法„„„„„„„„„„„„„„„12实验结果„„„„„„„„„„„„„„„„29分析与讨论„„„„„„„„„„„„„„„38小结„„„„„„„„„„„„„„„„42参考文献„„„„„„„„„„„„„„„43附录„„„„„„„„„„„„„„„„„48致谢„„„„„„„„„„„„„„„„„49综述„„„„„„„„„„„„„„„„„50正文„„„„„„„„„„„„„„„„„50参考文献„„„„„„„„„„„„„„„„54独创性声明„„„„„„„„„„„„„„„„

3、572温州医科大学硕士学位论文中英文缩略词表英文缩写英文全称中文名称FAFolicacid叶酸ASAtheroseleosis动脉粥样硬化VSMCvascularsmoothmusclecell血管平滑肌细胞PDGF-BBPlatelet-derivedgrowthfactor-BB血小板衍生生长因子-BBRAPARapamycin雷帕霉素IL-6Interleukin-6白介素-6IL-1βInterleukin-1β白介素-1βTNF-αTumornecrosisfactor-α肿瘤坏死因子-αα-SMASmoothmuscleα-acin平滑肌-α肌动蛋白

4、CHDCoronaryatheroscleroticheartdisease冠状动脉粥样硬化性心脏病LDLR-/-LDLreceptorknock-outLDLR基因敲除SPFSpecificpathogenfree无特定病原体TCTotalcholesterol总胆固醇TGTriglyceride甘油三酯LDL-CLowdensitylipoproteincholesterol低密度脂蛋白胆固醇VLDL-CVerylowdensitylipoprotein极低密度脂蛋白胆固醇cholesterolHDL-CHighdensitylipoproteinchole

5、sterol高密度脂蛋白胆固醇MDAMalondialdehyde丙二醛SODSuperoxidedismutase超氧化物歧化酶GSH-PxGlutathioneperoxidase谷胱甘肽过氧化物酶mTORMammaliantargetofrapamycin哺乳动物雷帕霉素靶蛋白ELISAEnzyme-linkedimmunosorbentassay酶联免疫吸附测定法HEHematoxylin-eosinstaining苏木素-伊红染色BCABicinchoninicacid二喹啉甲酸DMEMDulbecco'smodifiedeaglemedium细胞培养

6、基PAGEPolyacrylamidegelelectrophoresis聚丙烯酰胺凝胶电泳SDSSodiumdodecylsulfate十二烷基磺酸钠BSABovineserumalbumin牛血清白蛋白PBSPhosphatebuffersaline磷酸盐缓冲液3温州医科大学硕士学位论文DMSODimethylsulfoxide二甲基亚砜PMSFPhenylmethylsulfonylfluoride苯甲基磺酰氟丝氨酸蛋白酶的抑制剂TBSTris-buffered-saline洗膜缓冲液PVDFPolyvinylidenefluoride聚偏二氟乙烯TBST

7、Tris-buffered-salinewithtween加吐温的洗膜缓冲液TEMEDTetramethylethylenediamine四甲基乙二胺APSAmmoniumpersulfate过硫酸铵HRPHorseradishperoxidase辣根过氧化物酶ECLEnhancedchemiluminescence增强化学发光ICCImmunocytofluorescent细胞免疫荧光FITCFluoresceinIsothiocyanate异硫氰酸荧光素ODOpticaldensity光密度4温州医科大学硕士学位论文叶酸抑制血管平滑肌细胞去分化的分子机制研究

8、中文摘要目的:探究叶酸在