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1、作者简介：李粮辉(1990-)，女，硕士，研究方向：心肌保护，E-mail:huixiaoyi68@163.com通讯作者：陈文华，男，教授、研究生导师，E-mail:whc6202@163.com大鼠心肌缺血再灌注两种不同TTC染色方法的比较李粮辉1，陈文华1，郑宏2（1.福建医科大学附属协和医院麻醉科，福州，350001；2.南京军区福州总医院麻醉科，福州，350025）【摘要】目的：比较应用不同2,3,5-氯化三苯基四氮唑(TTC)染色方法对大鼠心肌缺血再灌注损伤后梗死面积的检测效果。方法：将20只SD大鼠(雄性
2、、8周龄，体重250～300g)按随机数字表法分为两组，每组10只，A组：传统TTC染色法组，B组：改进后的TTC染色法组，分别进行大鼠心肌染色,随后计算心肌梗死面积及测定血清cTnI浓度水平。结果：A组和B组均能较好地标记梗死心肌;A组和B组心肌梗死面积百分比无统计学差异（48.69±5.37%VS47.41±3.28%，P>0.05）；A组和B组血清cTnI浓度水平无统计学差异（4.51±0.88ng/mlVS4.70±0.71ng/ml，P>0.05）；但B组心肌切片染色色泽对比度及心肌非梗死区与梗死区区分度均高
3、于A组。结论：改进后的心肌TTC染色法采用在体染色，不仅操作简便，节省了实验时间和经费，而且提高了染色效果，能更准确地反映心肌缺血再灌注损伤的程度。因此改进后的心肌TTC染色法是一种经济、简便、快捷、高效的染色方法。【关键词】缺血再灌注;心肌梗死面积;2，3，5-氯化三苯基四氮唑;染色AComparisonofTwoDifferentTTCStainingMethodsforIschemia-reperfusionMyocardiuminRatsLILiang-hui1，CHENWen-hua1，ZHENGHong2(
4、1.DepartmentofAnesthesiology,Fujianmedicineuniversityaffiliatedxiehehospital,Fuzhou350001,China;DepartmentofAnesthesiology,FuzhougeneralhospitalofNanjingmilitarycommand,Fuzhou350025,China)【Abstract】ObjectiveTocomparethedifferentTTCstainingmethodsofmeasuringmyoca
5、rdialinfarctsizeafterischemia-reperfusioninrats.Methods20RatswererandomlydividedintotwogroupsincludinggroupAwithtraditionalTTCdyeingmethodandgroupBwiththemodifiedTTCdyeingmethodforischemia-reperfusionmyocardium,theninfractsizewascaculatedandthelevelsoftheirserum
6、cTnIweredetermined.ResultsBothgroupAandgroupBdetectedtheinfarctedmyocardiumwell;therewerenosignificantdifferenceinthemyocardialinfarctsizebetweengroupAandgroupB（48.69±5.37%VS47.41±3.28%，P>0.05）;therewerenosignificantdifferenceinthelevelsofserumcTnIbetweengroupAa
7、ndgroupB（4.51±0.88ng/mlVS4.70±0.71ng/ml，P>0.05）;butcomparewithA,thecolourcontrastofdyedmyocardialsliceandthedifferentiationofinfarctionareaandnon-infarctionareaweremuchcleareringroupB.ConclusionsThemodifiedTTCdyeingmethodusingInvivostainingisakindofeconomic,conv
8、enient,fastandefficientmethodwithbeingeasytocontrol,savingexperimentaltimeandexpense,improvingthedyeingeffects,evaluatingthesizeofmyocardialischemia/reperfusioninjury
