黄芩素对大鼠心肌缺血再灌注损伤心肌酶的影响

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1、黄芩素对大鼠心肌缺血再灌注损伤心肌酶的影响#黄勇攀1,2，叶兰1，石刚刚2*510（1.贵阳医学院药理学教研室，贵阳550004；2.汕头大学医学院药理学教研室，汕头515041）摘要：目的研究黄芩素对大鼠心肌缺血再灌注损伤保护作用的影响。方法：大鼠冠状动脉左前降支结扎30min后恢复血液灌注30min，于缺血前舌下静脉注射不同剂量黄芩素（40mg/kg，80mg/kg，160mg/kg）。测定血清CK、CK-MB、LDH、HBDH、GOT以及SOD活性、MDA的含量；观察心肌病理形态学改变。结果：黄芩素能降低由于缺血再灌注引起的心肌损害

2、心肌酶CK、CK-MB、LDH、HBDH、GOT的释放，保护SOD的活性，降低脂质过氧化物MDA的产生，并呈剂量效应关系。结论：黄芩素对大鼠心肌缺血再灌注损伤具有保护作用。关键词：药理学；黄芩素；心肌缺血再灌注损伤；肌酸激酶同工酶MB；超氧化物歧化酶；丙二醛中图分类号：R96615EffectsofBaicaleinontheenzymesofmyocardialtissueofmyocardialischemia-reperfusioninjuryinratsHUANGYongpan1,2,YELan1,SHIGanggang2(1.Dep

3、artmentofPharmacology,GuiyangMedicalUniversity,Guiyang550004;20252.DepartmentofPharmacology,ShantouUniversityMedicalCollege,Shantou515041)Abstract:Objective：Toinvestigatetheprotectiveeffectsofbaicalein（Bai）onratheartischemia-reperfusioninjurycausedbycoronaryarteryligation.

4、Methods:RatheartIPRinjurywasinducedbyoccludingtheleftanteriordescendingcoronaryarteryfor30minutesandrestoringbloodperfusionfor30minutes.Threedosages(40mg/kg，80mg/kg，160mg/kg，respectively)ofBaiwereintravenouslyinjectedbeforeheartischemia.Plasmacreatinekinase(CK),creatinekina

5、seisoenzymeB(CK-MB),lactatedehydrogenase(LDH),α-Hydroxybutyratedehydrogenase(HBDH),grutamicoxalacetictransaminase(GOT),malondiadehyde(MDA)concentrationsandsuperoxidedismutase(SOD)activityweremeasured.ThepathologicchangesofI/Rinjurywereobservedonthemicroscopy.Results:：Baire

6、ducedthereleaseofCK,CK-MB,LDH,HBDHandGOTfromI/Rrat30354045hearts,increasedtheactivityofSODanddecreasedtheMDAproduct.InBai(1mg·kg-1)group,theserumCK-MB,LDHandHBDHconcentrationswereloweredsignificantly(vsI/RgroupP<0.05).InBai(40,80,160mg/kg)significantly(vsI/RgroupP<0.05),an

7、dSODactivityincreasedsignificantly(vsI/RgroupP<0.05).Thedecreasegroups,serumCK,CK-MB,LDH,HBDHandGOTconcentrationsandMDAcontentweredecreasedofCK,CK-MB,LDH,HBDHandMDAinBai(80,160mg/kg)groupwasmoreremarkablethanthatinBai(40mg/kg)group(P<0.01).CK-MBinBai(40mg/kg)groupwaslowerth

8、anthatinVerapamil(2mg/kg)group(P<0.05).Formorphology,myocytesofI/Rheartshowedintra