naadp通过二孔通道对大鼠肺动脉平滑肌细胞内钙离子浓度及“钙火花”影响

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1、广州医学院博士学位论文广州医学院2008级博士学位论文NAADP通过二孔通道对大鼠肺动脉平滑肌细胞内钙离子浓度及“钙火花”的影响2+NAADPMediates[Ca]IandCalciumSparksinPulmonaryArterySmoothMuscleCellsviaTwoPoreChannels研究生：蒋永亮导师：冉丕鑫教授专业：呼吸内科学广州医学院·广州2012年4月2广州医学院博士学位论文TABLEOFCONTENTSListofAbbreviations.............................

2、.................................4AbstractinEnglish………………………………….............5AbstractinChinese………………………………………….14Introduction………………………………………………….21Materialsandmethods………………………………………33Results……………………………………………………….61Discussion…………………………………………………...82Conclusion………………

3、…………………………………...87Publications……………………………………….................993广州医学院博士学位论文ListofabbreviationsIP31,4,5-trisphosphateEREndoplasmicreticulumIP3R1,4,5-trisphosphatereceptorsRyRsRyarodinereceptorscADPRCyclicadenosinediphosphateriboseNAADPNicotinicaciddinucleotidephosp

4、hateTPCsTwo-porechannelsPASMCsPulmonaryarterialsmoothmusclecellsET-1Endothelin-1PLCPhospholipaseCRT-PCRReverse-transcriptionpolymerasechainreactionVOCCsVoltage-operatedcalciumchannelsROCCsReceptor-operatedcalciumchannelsSOCCsStore-operatedcalciumchannelsNADPNicotin

5、amideadeninedinucleotidephosphateCICRCalcium-inducedcalciumrelease4广州医学院博士学位论文2+NAADPmediates[Ca]IandcalciumsparksinpulmonaryarterysmoothmusclecellsviatwoporechannelsPh.Dcandidate:YongliangJiangMentor:PixinRanAbstract1BackgroundNotonlyaretherepreprogrammedpathsform

6、ostcells,buttheever-changingenvironmentalsoprovidesinstructionssothatthecellscanadjusttheirgrowthpatternsandactivitiesandultimatelymakelifeordeathdecisions.Cellsarethusexposedtobothintrinsicandextrinsicsignalsintheformofchemicaland/orphysicalfactorsthatarepresented

7、tothemfromeithersideoftheplasmamembrane.Thesesignalsareconvertedintointracellularmessengersthroughtheactionsofreceptors,ionchannels,2+and/oreffectorenzymes.Amongthesemessengers,calciumions(Ca)are2+offundamentalimportancetosignaltransductioninanimalcells.Ca-releasef

8、romintracellularstoresisafundamentalcellularprocessthatisinvolvedinthecontrolofnumerousphysiologicalfunctionsincludingmusclecontraction,secretion