柴胡皂苷a与黄芩素配伍抗人肺癌细胞a549、h460体外作用的实验研究

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1、河南中医学院２０１3届硕士研究生学位论文柴胡皂苷A与黄芩素配伍抗人肺癌细胞A549、H460体外作用的实验研究研究生姓名：李璐导师：王宪龄教授指导组成员：曹明卓老师学科、专业：中药学所属院、部：药学院中国﹒郑州２０１３年4月３０日目录摘要...............................................................................................................1Abstract......................

2、....................................................................................2前言...............................................................................................................4材料和方法.........................................................

3、...........................................81材料....................................................................................................81.1细胞系.......................................................................................81.2试剂及药品...........

4、.....................................................................81.3仪器...........................................................................................91.4溶液试剂及药液的配制方法........................................................91.5器具的清洗与灭菌...........

5、.........................................................101.6盖玻片的清洗与灭菌.................................................................101.7细胞培养..................................................................................102方法.............................

6、.....................................................................112.1四唑盐(MTT)比色法检测人肺癌A549和H460细胞增殖.............112.2流式细胞仪(FCM)检测人肺癌A549细胞和H460细胞凋亡.........122.3流式细胞仪(FCM)检测人肺癌A549和H460细胞周期................132.4免疫细胞化学法检测人肺癌A549细胞Bcl-2、caspase-3和cox-2表达......

7、............................................................................................133统计学分析........................................................................................14结果.....................................................................

8、........................................151柴胡皂苷A、黄芩素及其配伍诱导A549细胞及H460细胞后形态学观察151.1复苏传代后A549细胞形态........................................................151.2柴胡皂苷A、黄芩素及其配伍诱导A5