以壳聚糖为载体新型免疫亲和吸附剂与分子印迹聚合物的制备与应用分析

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1、以壳聚糖为载体免疫亲和吸附剂及分子印迹聚合物的制备及其应用研究recoveryratesinanimaltissue．feedwerefrom78．44％to91．09％withCVlessthan6．46％．2．Themolecularlyimprintedpolymers(MIPs)selectiveforMAwerepreparedbyreversesuspensionpolymerizationusingchitosanasfunctionalmonomerandepichlorohydrinby

2、ascross-linker．Theoptimumconditionsforpreparationwerefoundasfollows：thebestratioofchitosantoMAwas6：1，pHof9．0，sucroseof2％，epichlorohydrinbyconcentrationof20％，theratioofmethanoltowaterwas1：2．TheFouriertransformIRspectroscopyandScanningElectronicMicroscopewe

3、reusedtocharacterizethesurfacestructureandthephysicochemicalpropertiesofthesorbents．Theefficiencyofloading，washingandeluentsolutionwerebestusing10％methanol-watersolution，distilledwater，4mLmethanolrespectively．Themaximumbindingcapacityofthemolecularlyimpri

4、ntedsolid-phaseextraction(MISPE)reached11．5mg／gwetadsorbent．andtherearetwodifferentbindingsiteswerefoundedbetweenMAandMIPsbythedynamicadsorptionanalysis．Theaveragerecoverywas95．94％withtheCVamongcolumnsof2．90％．After18timesofrepeateduse，therecoveryrateandco

5、lumncapacitybothstillremainedover90％．Thecalculationoftheseparationfactors(p)ofCHI—MIPsshowedthattheimprintedmatrixwasspecificandapproximately10timesgreaterthantheCHI—NIPs．TherecoveriesofMAfrompork，liver，feed，eggswereintherangeof94．26％一106．86％withtheCVless

6、than4．68％．KEYWORDS：Immunosorbents，Molecularlyimprintedpolymers，Chitosan，Methandrostenolone，HPLC江苏大学硕士学位论文目录第一章绪论⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．11．1农、兽药残留检测的现状⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯11．1．1农、兽药残留检测技术与方法⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．．11．1．2样品净化处理技术与方法⋯⋯⋯⋯⋯⋯⋯

7、⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯。21．2壳聚糖⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯91．2．1壳聚糖的理化性质⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．91．2．2壳聚糖的应用⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯101．3去氢甲睾酮⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯。111．4立题的依据与意义⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．121．5论文的主要研究内容⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．12第二

8、章以壳聚糖为载体去氢甲睾酮免疫亲和柱的制备及应用研究⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．132．1引言⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．．132．2试剂与仪器⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．132．2．1主要试剂⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯132．2．2主要仪器设备⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯142．3试验方法⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯