木犀草素分子印迹聚合物微球的制备与应用.研究

木犀草素分子印迹聚合物微球的制备与应用.研究

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时间:2019-01-30

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1、AbstractMolecularimprintingtechniqueisanewtechnologywhichtheprinciplesimulatetotheinteractionbetweenantigenandantibody.Settingthetargetmoleculewhichneedtoseparateastemplate,throughthepolymerizationreactiontogetakindofpolymerwhichhavespecificselectivetoaparticularmatter.Thepreparationprocessea

2、sytocontrol,andpropertyissteady.Now,thetechnologyiswidelyused.Naturalproductsactiveingredientsisamainsourcewhichcouldpreventandcurethediseaseofhuman,andtheactiveingredientisbasisofthefunctionmatter.Luteolinisakindofflavonoidcompound,Thereareavarietyofpharmacologicalactivity,suchasantitumor,an

3、tivirus,oxidationresistance,fightbacteriumdiminishinflammation,etc.Peanutshellsisakindofcheapandrenewableabundantnaturalresource.Luteolin,amajorflavonoidinpeanuthull.Throughtheextractionandseparationtogethighpurityofluteolin,whichcouldbeusedforrawmaterialfordrugsorhealthfoods,havingabroadappl

4、icationprospectinthemarket.Thecontentsofthispapermainlyincludeaslist:1.Luteolinwasusedastemplatemolecule,undertheactionoffunctionalmonomerandcrosslinkingagent,addpolyethyleneglycol2000asdispersant,tolueneasporogenicagent,synthetizedluteolinmolecularlyimprintedpolymermicrospheresinthecondition

5、ofaqueoussolution.Thestructureofmolecularimprintingmicrosphereswerecharacterizedbyinfraredspectrometer,laserparticleanalyzer,Liquidnitrogenadsorptioninstrument,ect.Examinedthepreparationconditions’effecttotheadsorptionquantityandpolymermorphology,gainingthebetterpreparationconditions.2.Thesta

6、ticadsorptivemethodwasusedtoanalysetheadsorptionperformanceofluteolininthemolecularlyimprintedpolymermicrosphere.Theisothermaladsorptionindicatedthatthebiggestadsorptionquantitywasdifferentbetweenimprintedandnotimprintedmolecularlyimprintedpolymermicrospheres.Accordingtoscatchardequationanaly

7、siscouldbeinformedthat:incertainconcentrationrange,molecularlyimprintedpolymermicrosphereformedtwodifferentbindingsites.Throughcalculatingthebalanceequationofdissociationconstants,theresultswere0.2198mmol/Land2.7603mmol/L.Theisothermaladsorpt

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