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1、入门指南简介和相对定量相对定量实验示例实验美国应用生物系统公司7300/7500实时定量PCR仪设计相对定量实验PrimerExtendedonmRNA5′3′执行反转录5′cDNAReversePrimerOligod(T)orrandomhexamerSynthesisof1stcDNAstrand3′5′cDNA从相对定量反应板生成PCR数据在相对定量研究中生成PCR数据©Copyright2004,AppliedBiosystems.Allrightsreserved.ForResearchUseOnly.Notforuseindiagnosticprocedures.Authoriz
2、edThermalCyclerThisinstrument,SerialNo___________,isanAuthorizedThermalCycler.Itspurchasepriceincludestheup-frontfeecomponentofalicenseunderUnitedStatesPatentNos.4,683,195,4,683,202and4,965,188,ownedbyRocheMolecularSystems,Inc.,andundercorrespondingclaimsinpatentsoutsidetheUnitedStates,ownedbyF.Hoff
3、mann-LaRocheLtd,coveringthePolymeraseChainReaction("PCR")processtopracticethePCRprocessforinternalresearchanddevelopmentusingthisinstrument.TherunningroyaltycomponentofthatlicensemaybepurchasedfromAppliedBiosystemsorobtainedbypurchasingAuthorizedReagents.ThisinstrumentisalsoanAuthorizedThermalCycler
4、forusewithapplicationslicensesavailablefromAppliedBiosystems.ItsusewithAuthorizedReagentsalsoprovidesalimitedPCRlicenseinaccordancewiththelabelrightsaccompanyingsuchreagents.PurchaseofthisproductdoesnotitselfconveytothepurchaseracompletelicenseorrighttoperformthePCRprocess.Furtherinformationonpurcha
5、singlicensestopracticethePCRprocessmaybeobtainedbycontactingtheDirectorofLicensingatAppliedBiosystems,850LincolnCentreDrive,FosterCity,California94404.DISCLAIMEROFLICENSE:Norightsforanyapplication,includinganyinvitrodiagnosticapplication,areconveyedexpressly,byimplicationorbyestoppelunderanypatentor
6、patentapplicationsclaiminghomogeneousorreal-timedetectionmethods,includingpatentscoveringsuchmethodsusedinconjunctionwiththePCRprocessorotheramplificationprocesses.The5'nucleasedetectionassayandcertainotherhomogeneousorreal-timeamplificationanddetectionmethodsarecoveredbyUnitedStatesPatentNos.5,210,
7、015,5,487,972,5,804,375and5,994,056,ownedbyRocheMolecularSystems,Inc.;bycorrespondingpatentsandpatentapplicationsoutsidetheUnitedStates,ownedbyF.Hoffmann-LaRocheLtd;andbyUnitedStatesPatentNos.5,538,84
