PEDV野毒株和疫苗毒株多重RT-PCR鉴别检测方法的建立及应用

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1、分类号：密级：HUAZHONGAGRICULTURALUNIVERSITY硕士学位论文MASTER’SDEGREEDISSERTATIONPEDV野毒株和疫苗毒株多重RT-PCR鉴别检测方法的建立及应用ESTABLISHMENTANDAPPLICATIONOFMULTIPLEXRT-PCRMETHODFORIDENTIFICATIONOFPEDVWILDANDVACCINESTRAINS研究生:何东贤CANDIDATE:HEDONGXIAN学号:2016302120106STUDENTN:专业:兽医

2、硕士MAJOR:MASTERDEGREEINVETERINARYMEDICINE导师:何启盖教授SUPERVISOR:PROFESSORHEQIGAI汤细彪博士DOCTORTANGXIBIAO中国武汉WUHAN,CHINA二〇一八年六月JUNE,2018华中农业大学硕士学位论文PEDV野毒株和疫苗毒株多重RT-PCR鉴别检测方法的建立及应用EstablishmentandApplicationofMultiplexRT-PCRMethodforIdentificationofPEDVWildandV

3、accineStrains研究生:何东贤学号:2016302120106指导教师:何启盖教授汤细彪博士学位类型:兽医硕士领域：华中农业大学动物医学院中国·武汉CollegeofVeterinaryMedince,HuazhongAgriculturalUniversityWuhan,ChinaPEDV野毒株和疫苗毒株多重RT-PCR鉴别检测方法的建立及应用目录摘要.....................................................................

4、...............................................................iAbstract...............................................................................................................................iii缩略语表(Abbreviation)................................

5、......................................................................v1前言..................................................................................................................................11.1猪流行腹泻病毒(PEDV)...............................

6、............................................................21.1.1PEDV的病原学特征..........................................................................................21.1.2PEDV的基因组结构......................................................................

7、....................31.1.3PEDV蛋白结构和功能......................................................................................41.1.3.1复制酶多聚蛋白...........................................................................................41.1.3.2S蛋白............

8、................................................................................................41.1.3.3ORF3蛋白....................................................................................................41.1.3.4E蛋白...............