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1、多发性骨髓瘤患者外周血单个核细胞c-Cbl和Cbl-b基因表达下降方苏1，李萡1*，牛宇哲1,2，陈少华1，杨力建1，李扬秋1,2（510632广州，暨南大学：血液病研究所1;再生医学教育部重点实验室2）[摘要]目的了解多发性骨髓瘤（multiplemyeloma，MM）患者外周血单个核细胞中c-Cbl和Cbl-b基因表达水平。方法利用SYBRGreen实时定量PCR方法，检测16例MM患者和12例健康人外周血单个核细胞c-Cbl和Cbl-b基因的表达情况，以β2微球蛋白基因(β2M)作为内参照，采用2-ΔCt×100%计算c-Cbl和Cbl-b基因相对表达水
2、平。结果MM患者中c-Cbl和Cbl-b基因表达水平与健康人相比均下降，其中MM组c-Cbl(平均秩：12.56)表达水平与健康对照组（平均秩：17.08）比较无统计学意义（P=0.159）；而Cbl-b（平均秩：10.72）表达水平与健康对照组（平均秩：19.54）则显著降低（P＜0.05﹚。结论c-Cbl和Cbl-b表达下调可能通过影响BCR和TCR信号传导通路从而影响机体的免疫调节功能。[关键词]多发性骨髓瘤（MM）；c-Cbl；Cbl-b；荧光定量PCR［中图法分类号］［文献标志码］ADecreasedc-CblandCbl-bgenesexpress
3、ioninperipheralbloodmononuclearcellsfrommultiplemyelomapatientsFangSu1,LiBo1*,NiuYuzhe1,2,ChenShaohua1,YangLijian1,LiYangqiu1,2(JinanUniversity1.InstituteofHematology;2.RegenerativeMedicine,MinistryofEducationKeyLaboratory,Guangzhou,Guangdongprovince,510632,China)［Abstract］Objective
4、Toinvestigatetheexpressionlevelofc-CblandCbl-bgenesinperipheralbloodmononuclearcellsofpatientswithmultiplemyeloma（MM）.MethodsTheSYBRGreenquantitativereal-timePCRwasusedfordetectingc-CblandCbl-bgenesexpressionlevelinperipheralbloodmononuclearcells（PBMC）of16patientswithMMand12healthyi
5、ndividuals,β2-microglobulingene(β2M)wasusedasanendogenousreference.Relativechangesofc-CblandCbl-bgenesexpressionlevelwereusedbythe2-ΔCt×100%method.ResultsDecreasedc-CblandCbl-bgenesexpressionlevelwerefoundinMMpatients,therenosignificantdifferenceofc-CblgeneexpressionlevelbetweenMMpa
6、tients(averagerank：12.56)andhealthycontrol（averagerank：17.08）（p=0.159）.However,theexpressionlevelofCbl-bgeneinMM（averagerank：10.72）wassignificantlylowerthanthatinhealthycontrol（averagerank：19.54）（P＜0.05﹚.ConclusionInthisstudy,wefirstlyreportedthechangeofc-CblandCbl-bgenesexpressionl
7、evelinMMpatients.Thedown-regulatedexpressionlevelofc-CblandCbl-bgenesmayleadtoabnormalityofBCRandTCRsignalingpathwaywhichmaybeaffecttheimmuneregulatoryfunction.[Keywords]:multiplemyeloma（MM）;c-cbl;cbl-b;quantitativereal-timePCRSupportedbytheNationalNaturalScienceFoundationofChina(30
8、972455),Correspondi
