atra对人结直肠癌lovo细胞sicam-1表达的影响

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1、中山大学硕士学位论文ATRA对人结直肠癌LoVo细胞sICAM—l表达的影响Whenthecellwasindena叫logarithmgro州hcondition，RPMI-l640、Vastransformedtocontrolgroup，andRPMI—l640withcorrespondingall—transretinoicacidconcentrationwastmnsf’ornledtocorrespondinggroup．5串l06cellsofdi行erenta11．transretinoicacidconc

2、entrationweretransferedto5mlRPMI．1640tocuIturef．0r24h，48h，72hontheconditionof370C、5％C02saturatedhumidity．Thentheculture-liquidwaScollected，centri向gedat2000r／minforlOminutes，andstoredatat一20℃．DotheELISAexperiment，measureOD—Valuein450nmwaVelen舀hofeachgroup．Eachsamplei

3、stestedtwice，andgettheaVerageValue．LaStly，puta11ofthedatainto“SPSS11．0forWindows”soRware，dealwith“T’WoWayANOVA”，atlastdrawthegraphsofinhibitionratio．ResuItsI．TheexpressionofsICAM—lofalcoholgrouphadnostatisticaldif诧rencecomparingwithcontrolgroup(p>O．05)．Itwascleartha

4、tl0。2mol／Lalcohol，thesolventofall-transretinoicacidhadnoe髋ctontheexpressionofsICAM·lofLo、，ocelI．Buttherewerestatisticaldiff．erencebetweenlO。5mol／La11．tmnsretinoicacidgroup，10．6mol／La11．transretinoicacidgroupand10‘7mol／Lall—tmnsretinoicacidgroupcompa—ngwithcontrolgro

5、up(p<0．05)，andthedi艉renceamongthethreedi虢rentconcentrationalI—transretinoicacidgrolJpalsohadstatisticaldi能rence(p

6、h91．0upindiff-erentperiodshowedthattheexpressionofsICAM一1ofLoVbcell、^，asinhibitedbyall—t啪sretinoicacidinadose—andtime—dependentmanner．Conclusion：all-tmnsretinoicacidcaninhibittheexpressionofsICAM-lofLoVIocellinadose—andtime—dependentmanner．Thecellinhibitionratioincr

7、eaSedwiththeincreasingoftheconcentmtionofa11．tmnsretinoicacidandprolongingoftime．ConcIusionslTheLoVroceIlsexpressessICAM·1．2All—transretinoicaCidisabletoinhibittheexpressionofsICAM—linhumanCRCL01Vrocells，andshowinadose—andtime—dependentmanner．3All-transfetinoicacidi

8、sablet0inh．bittheexpressionofsICAM—linhumanCRCLoVoceIls，andaccommodatebodyimmunity．Soall—tmnsretinoicacidisabletoinhibitthemetastasisofthe