滁州鲫雌核发育的细胞学研究

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1、安徽农业大学学报，2009，36(4)：533—537JournalofAnhuiAgrl’culturalUniversity滁州鲫雌核发育的细胞学研究徐广友，万全，袁兆祥，马明发，凌武海，吴泊君(1．滁州市水产技术推广中心站，滁州239000；2．安徽农业大学动物科技学院，合肥230036；3．滁州市渔场，滁州239000；4．滁州市水产研究所，滁州239000)摘要：研究产于安徽省滁州市城西水库的滁州鲫是否行雌核发育繁殖。对滁州鲫辛×滁州鲫5及滁州鲫早X红鲤6受精卵进行细胞学研究，结果表明：(1)在刚产出的成熟卵细胞内没有发现极体，在

2、精子入卵约l5—16min后(水温为18—20~C)，单个极体被排出；(2)在受精卵内，精核保持凝聚状态，没有形成雄性原核；(3)精子入卵20min后，卵核形成雌性原核，在第一次卵裂前没有观察到雌性原核与呈凝聚状态的精核的融合；(4)同源与异源精子激发滁州鲫卵的发育过程基本相同，异源精子能进入滁州鲫卵细胞并激发卵核形成雌性原核，并进行正常胚胎发育，其鱼苗正常，无畸形和无杂交性状的鱼苗。研究表明，滁州鲫为雌核发育鱼类。关键词：滁州鲫；红鲤；雌核发育；细胞学中图分类号：$965．117文献标识码：A文章编号：1672—352X(2009)04-

3、0533-05AcytologicalstudyongynogenesisofChuzhouCruciancarp(Carassiusauratus)xuGuang．you，WANQuan，YUANZhao．xiang，MAMing—fa，LINGWu—hai，WUBo-jun(1．ExtensionStationofAquiculturalTechniqueinChuzhou，Chuzhou239000；2．SchoolofAnimalScienceandTechnology，AnhuiAgriculturalUniversity，Hef

4、ei230036；3．FishFarmofChuzhouCity，Chuzhou239000；4．ChuzhouFisheriesResearchInstitute，Chuzhou239000)Abstract：ThepurposeofourinvestigationwastoexaminewhethertheChuzhouCruciancarpcaughtfromCity—westreservoirinChuzhouCity，AnhuiProvince，wouldreproducebygynogenesis．Theirspawnswere

5、artificiallyin-seminatedwithmihofCrueiancarpandRedcarp(CyprinuscarpioL．redvariety)，andanumberoffertilizedeggswerestudiedcytologicallybeforetheirfirstcleavages．Theresultsofobservationareasfollows：(1)Nopolarbodywasfoundintheripeeggcellsjustafterspawning．About15minutesafterin

6、semination(at18～20~Cwater)，asinglepolarbodywasextruded．(2)Inthefertilizedeggs，thespermnucleusremainedasacondensedmassanddidnottransformintomalepronucleus．(3)Afterthesinglepolarbodywasextruded，about20minutesafterinsemina。tion，theeggsnucleustransformedintofemalepronucleus，bu

7、tbeforetheirfirstcleavagesnoamphimixiswasob—served．(4)TheheterologousspermcouldnormallyentertheeggcytoplasmofChuzhouCruciancarpandactivatefemalepronucleusformationandnormaldevelopmentintoembryos．Theresultsshowedthateitherhomologousorho—teroloousspermnucleushadthesamedevelo

8、pmentalprocess．Thefrydevelopednormallyandnodeformedfryandhybridizationfeatureswerefound．A