干细胞分化及其对急性肝衰竭大鼠治疗的实验研究

《干细胞分化及其对急性肝衰竭大鼠治疗的实验研究》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。

1、第三军医大学博士学位论文干细胞分化及其对急性肝衰竭大鼠治疗的实验研究姓名：周一鸣申请学位级别：博士专业：内科学（传染病学）指导教师：胡大荣20050501第三军医大学博士学位论文英文缩写2-AAF2-acetylaminofluoreneABCavidin··biotin--peroxidasecomplexAFPALBBSACcl4CFUFCKCKl9DABp琶PCDAPIDMEMDPpIVEDTAEGFFACSfAHAlpha-fetoproteinAlbuminBoveinserumalbuminTetrachlorideClonyformingunitsfibroblast

2、sCytokineCytokeratin19DiaminobenzidineDiethylpyrocarbonate4’，6--Diamidino一2一phenylindoledihydrochloridehydrateDulbecco'smodifiedEaglelSmediumDipetideldipeptidaseIVEthylenediaminetetraceticacidEpidermalgrowthfactorFtuorescerrce-ac6vatedcellsortingFumarylacetoacetatehydrolaseFCSFetalcalfserum粥FF

3、ibroblastgrowthfactorGAPDHGlyceraldehyde一3一phosphateEGFPdehydrogenaseEnhancedgreenfluorescentprotein2一乙酰氨基芴抗生物素一生物素一过氧化物酶复合物甲胎蛋白白蛋白牛血清白蛋白四氯化碳成纤维细胞集落形成单位细胞因子绥瑰热罄自193，3一二氦基苯联胺焦磷酸二乙酯4’，6一二醚基一2一苯攀吲哚盐酸纯合饬培养液二肽酶IV乙二胺四乙酸表皮生长困予流式细胞仪延胡羧酰乙酰乙酸水鳃酶胎牛血清纤维缎戆生茨因子甘油醚～3一磷酸脱氢酶增强型绿色荧光蛋自第三军躐太学博士学位论文HGFHPeHSCMSCsNP

4、eSOCOVPBSPCRHPPVDFRT．PCRSCFSRY稻￡8TNF．晓VEGFX-galHepatocytesgrowthfactorHepaticprogenitorcellsHepaticstellatecellsMarrowmesenchymalstemcellsNonparench)analOvaIcells0valcellmarkerPhosphate-bufferedsalinePolymerasechainreactionHepatectomypotyvinylidenedifluofideReversaltranscriptpolymerasechainrea

5、ctionStemcellfactotSex-determiningregionYchromosomeTransforminggrowthfactor-lSTumotnecrosisfactor-ctVascularendothelialgrowthfactor5-Bromo一4-chloro一3一indolyl-$一D—gatactoside脬纲胞生长困予捋手租细麓肝星细胞嚣髓闻充质干细胞jI于菲实质纲藏卵圆细胞卵圆细胞标志磷酸缓冲浚聚合酶链式反应jl干部分切除术聚偏二乙爝二穰酯逆转录PCR予缨旎蠢予Y染色体性别决定区转稔生长因子一#肿瘤坏死因子川赢管内皮生长隧子5．溪．4一氯一

6、3一甥绦一§一D一半魏糖苷第三军鬣太譬博士学位论文DifferentiationofstemcellsandtherapyoftransplantedstemcellsderivedfrommarrowfortheacuteliverdisfunctionofratsAbstractHumanhealthhasbeenthreatenedbadlybyliverdisease．Livertransplantationispresentlythesolecurativetherapyforhepaticfailure．Butitishighlycostlycomplexandlimi

7、tedbytheavailabili玲ofdonorlivers．Asanalternative，hepatocytetransplantationhasbeenconsideredwiththehopeofassistinghepaticregenerationinacutefailure。buttheshortlifeofisolatedhepatocytesandlimits的thetransplantablecellnumbersaswellasthesarn