人防御素α5、人防御素β2在不孕患者输卵管的表达及意义

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1、四川大学学报(医学版)2。10di)‘。；，4l(3)：●480—482JSichuanUniv(MedSciEdi人防御素I】【5、人防御素Ij；2在不孕患者输卵管的表达及意义胡诗婉，毛毳，张丹△，兰珍，王成芳，罗书四川大学华西第二医院生殖内分泌科(成都610041)【摘要】目的通过分析粘连、闭锁的输卵管伞和正常输卵管伞中人防御素a5(humanadefensin-5，H【)-5)、人防御素132(humanpdefensin-2，Hp2)的相关性，初步探讨HnI)_5和HI3D-2在输卵管伞粘连发生中的作用与意义，为临床防治输卵管伞粘连闭锁引起的不孕提供新的思路。方

2、法收集临床手术切除的输卵管伞组织，将输卵管伞分为粘连组(30例)和非粘连组(3O例)，应用免疫组化SP法检测HAD-5和H{ID-2的表达情况，分析二者的相关性。结果免疫组化染色结果显示HaI)-5、HflD-2两种因子在粘连组的表达强于不粘连组，且差异有统计学意义(P

3、键词】人防御素“5人防御素82免疫组织化学相关性【中图分类号】R711．76TheRelationshipBetweenHAD-5andHpD-2inInfertileWomen’SFimbriaeTubeswithAdhesionsandAtresiasHUShi-wan，MA0Cui，ZHANGDan，LANZhen，WANGCheng—fang，LUOShu．DepartmentofReproductiveEndocrinology，“ChinaSecondHospital，SichuanUniversity，Chengdu610041，China△Corresp

4、ondingauthor，E-mai1：zdzdokok@126．corn[Abstract]ObjectiveToinvestigatetheexpressionandrelationshipofhumanadefensin-5(HaD-5)andhuman8defensin-2(H~D-z)inhumanfimbriaetubeswithadhensionsandatresias．MethodsThetissuesampleswerecollectedfrom30humanfimbriaetubeswithadhensionsandatresias，and30cas

5、eswithoutadhensionsandatresias．TheexpressionofHaD-5andHI~D-2infimbriaetubetissueweremeasuredbyimmunohistochemicalSPmethods．Imagepro-plus6．0softwarewasusedtOtesttheaverageIODvalueofpositivestaining．DifferencesofHaC卜5andHi3D-2expressionswereanalyzedbyindependent—samplesTtest．Therelationshi

6、pbetweenHaI)-5andHflD-2wasanalyzedbyPearsoncorrelation．ResultsWefoundthatHdD一5andHJ3D-2mainlyexpressedintheepithelialcellswhichfaceto1umen，mostlyinthecytochylema．BothtWOgroupsexpressedHa5andHI3D-2，butthedegreesweredifferent．Comparedwithhumanfimbriaetubeswithoutadhesionsoratresias，theexpr

7、essionsofHaD·5andHflD-2increasedsignificantlyinadhesioncases(PHD一5—0．000，PHBD__2=0．02)．Inthegroupwithoutadhesion，therewasapositivecorrelationbetweenHaD’5andH~]D-2(r=0．404，P=0．027)，whileintheadhesiongroup，therewasnocorrelationbetweenHaD一5andH~D-2(P=0．089)．ConclusionHaD-5an