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《toll样受体9和核因子-κbp65在大鼠溃疡性结肠炎模型结肠组织中的表达及其相关性的研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、级UC大鼠相比P<0.05。6、UC大鼠结肠粘膜中TLR9表达与NF-κBp65表达呈显著正相关（rs=0.971，P<0.01）。结论：1、UC大鼠结肠粘膜中TLR9、NF-κBp65表达明显升高，且与疾病的组织学分级呈正相关，提示其可能与UC发生、发展有关。2、TLR9和NF-κBp65在UC结肠粘膜中的表达密切相关，呈显著正相关，TLR9可能促进NF-κBp65的表达。[关键词]：溃疡性结肠炎，大鼠，TLR9，NF-κBp656expressionoftolllikereceptor（ＴＬＲ）9andＮＦ-κＢp65incol
2、onicmucosaofratsmodelwithulcerativecolitisanditsrelationshipabstractObjective:ToinvestigatetheexpressionofToll-likereceptor9(TLR9)andNF-κBp65incolonicmucosaofratsmodelwithulcerativecolitis,toexploretheroleoftheminthecarcinogenesisofUC.AnalyzeitsrelationshipwithTLR9andN
3、F-κBp65.Methods:FortycleanandmatureSprague-Dawleyratswerestudiedinourexperiment,halfofthemarethemale,twentyratswereusedasmodelgroupatrandomamongthem,adoptedcompoundmethod(2,4-dinitrochlorobenzeneplusaceticacid)tomakemodelofratswithcellimmunoreactivityUC,theother20norma
4、lratswereusedascontrolgroup.Afterthemodelwasfinished,themacroscopicandhistologicalchangesofcolonicmucosawereobserverdandevaluated.ExpressionofTLR9andNF-κBp65incolonicmucosaweredetectedbyimmunohistochemicalElivisionmethod,andanalyzedtheirrelationshipwiththehistologicals
5、tage.SPSSversion14.0forwindowssoftwarewasusedforthestatisticalanalysis,usingχ2andSpearmanrelationanalysisinqualitativedata,SNK-qtestofOne-wayANOVAandttestinquantitativedata,Pvaluelessthan0.05wereconsideredstatisticallysignificant.Results:1.Afterfivetotendaysthemodelwas
6、finished,20ratsofmodelgroupbegantooccurthecommonsymptomsofUCsuchasdiarrhea,stenchstool,thinandnoncoagulativestool,andappearedtobloodystoolormucusandpurulentstool;hyperemia,hydrops,anobrosis,necrosisofcolonicmucosa,thickintestinalparietesandulcerationwasseenfromgrossapp
7、earanceofspecimen.AllofthemprovedtoaccordwiththestandardofUCbyhistologicaldiagnosis.Thesuccessfulrateofmakingmodelis100%;themacroscopicandhistologicalchangesofcolonicmucosaofmodelgroupwassignificantlyhigherthanthatofcontrolgroup,thedifferencehas7statisticalsignificance
8、(P<0.01).2.ThepositiveexpressionrateofTLR9incolonicmucosaofmodelgroupandcontrolgroupwere90%（18/20）and35%（7/20）respect
