芒果叶提取物—芒果苷联合麻黄碱对过敏性哮喘的增效减副作用研究

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1、学校代码：10600学号：101089学位类型：科学学位广西中医药大学硕士学位论文芒果叶提取物—芒果苷联合麻黄碱对过敏性哮喘的增效减副作用研究研究生：杜军导师：邓家刚教授院系（部所）：药学院专业：中药学研究方向：中药药效筛选与新药开发完成日期：2013年03月01日目录中文摘要··························1英文摘要··························3引言··························7正文··························9第一部分芒果苷联合麻黄碱治疗过敏性哮喘的药效学研究·····91实验材料

2、·························92实验方法························113实验结果························173.1给药后小鼠自主活动力的变化··············173.2激发阶段小鼠哮喘发作表现···············183.3对哮喘小鼠全血中白细胞总数及分类的影响········193.4对哮喘小鼠肺组织病理改变的影响············213.5对小鼠血清中OVA-sIgE含量的影响············223.6对小鼠血清中cAMP含量的影响··············234小结··

3、························24第二部分芒果苷联合麻黄碱及其联合用药对治疗过敏性哮喘的免疫分子机制初步研究··················251实验材料························252实验方法························273实验结果························303.1总RNA提取结果····················303.2芒果苷与麻黄碱对Th1/Th2型细胞因子mRNA表达影响····314小结··························34讨论············

4、·············351芒果苷联合麻黄碱治疗过敏性哮喘的药效学研究·······351.1芒果苷与麻黄碱联合用药的中医理论基础·········351.2哮喘模型的选择及验证·················371.3对哮喘小鼠自主活动力的影响··············381.4对哮喘小鼠气道炎症细胞的影响·············391.5对哮喘小鼠气道炎症介质IgE的影响···········411.6对哮喘小鼠气道cAMP的影响···············422芒果苷联合麻黄碱治疗过敏性哮喘的免疫分子机制初步研究···432.1Th1型细胞因子与哮喘····

5、··············432.2Th2型细胞因子与哮喘··················442.3Thl/Th2平衡与哮喘···················46结论·························48结语·························49参考文献·························50附录·························56综述·························58致谢·························71攻读学位期间发表的学术论文目录··············

6、72作者声明·························73个人简历·························74中英文缩略词汇表（abbreviations）缩写英文全称中文名称AHRairwayhyperresponsiveness气道高反应性cAMPcyclicAdenosineMonophosphate环磷酸腺苷DEPCdiethypyrocarbonate焦碳酸二乙酯EBethidiumbromide溴化乙啶EIAenzymeimmunoassay酶免疫分析试验ELISAenzyme-linkedimmunosorbentassay酶联免疫吸附试验EO

7、Seosinophil嗜酸性粒细胞HEhematoxylin-eosin苏木素伊红染剂IFN-γinterferon-gammaγ-干扰素IgEimmunoglobulinE免疫球蛋白EILinterleukin白细胞介素ODopticaldensity光密度/吸光度OVAovalbumin卵清蛋白OVA-sIgEovalbumin-specificimmunoglobulinEOVA特异性IgEPBSphosphatebufferedsaline磷酸盐缓冲液reversetranscriptionpo