布鲁氏菌外膜蛋白omp31在细胞凋亡中的调控研究

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1、分类号：密级：学号：2010109059单位代码：10759石河子大学硕士学位论文布鲁氏菌外膜蛋白OMP31在细胞凋亡中的调控研究学位申请人王慧指导教师王远志副教授申请学位门类级别医学硕士学科、专业名称免疫学研究方向感染免疫所在学院医学院中国·新疆·石河子2013年6月StudyontheapoptosisregulationofBrucellaOutMembraneProteinOMP31ADissertationSubmittedtoShiheziUniversityInPartialFulfillmentoftheRequirementsfo

2、rtheDegreeofMasterofMedicineByWanghui(Immunology)DissertationSupervisor:Associateprof.WangYuan-zhiJune,2013目录摘要................................................................................................................................ⅠAbstract.............................

3、...............................................................................................Ⅱ主要符号表........................................................................................................................Ⅲ引言.....................................................

4、.............................................................................1第一章..................................................................................................................................3羊种布鲁氏菌16M标准株omp31基因缺失株的构建及稳定性检测...........................31材料与方法

5、......................................................................................................................31.1材料........................................................................................................................31.2方法...........................

6、.............................................................................................42结果................................................................................................................................112.1同源重组质粒pGEM-7zf+-Δomp31-SacB的构建.................

7、...........................112.2SacB基因活性检测.............................................................................................162.3布鲁氏菌特异性检测..........................................................................................172.4同源重组的筛选..........................

8、.............................................................