夜香树提取物nocturnoside b体内外抗肝癌作用及机制研究

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1、学校代码：10600学号：101073学位类型：科学学位广西中医药大学硕士学位论文夜香树提取物NocturnosideB体内外抗肝癌作用及机制研究研究生：张明艳导师：赵世元副主任技师院系（部所）：药学院专业：药理学研究方向：中药抗肿瘤研究完成日期：2013年6月10日目录中英文缩略词汇表·········································1中文摘要·············································2英文摘要·····················

2、························4引言·············································7正文·············································9第一部分夜香树提取物NocturnosideB对人肝癌细胞株BEL-7404体外抗肿瘤作用的研究1材料··················································102方法··································

3、················123结果··················································194讨论··················································30第二部分夜香树提取物NocturnosideB抗人肝癌细胞株BEL-7404体内裸鼠移植瘤实验研究1材料··················································332方法··························

4、························333结果··················································374讨论··················································47结论················································51参考文献···············································52展望··················

5、······························56综述················································57致谢················································68攻读学位期间发表的学术论文目录····················69作者声明················································70参考文献······························

6、··················711中英文缩略词汇表英文缩写英文全称中文全称NOBNocturnosideBSSCNsteroidalsaponinsofCestrum夜香树甾体皂苷nocturnumNCSnewborncalfserum新生牛血清IRInhibitionrate细胞的增殖抑制率HCCHepatocellularcarcinoma肝细胞癌VEGFVascularendothelialGrowth血管内皮细胞生长因子factorDDPCisplatin顺铂NSnormalsaline生理盐水PBS

7、Phosphatebuffersaline磷酸盐缓冲液PIPropidiumiodide碘化丙啶HEHematoxylinEosin苏木精和伊红IL-8Interlukin-8白细胞介素8MVDMicrovesseldensity微血管密度IHCImmunohistochemistry免疫组织化学法TVTumorvolume肿瘤体积RTVRelativetumorvolume相对肿瘤体积PCNAProliferatingCellNuclearAntigen增殖细胞核抗原2中文摘要目的:探讨夜香树提取物Nocturno

8、sideB体外对人肝癌细胞BEL-7404以及体内对人肝癌细胞BEL-7404荷瘤裸鼠的抗肿瘤作用及其机制。方法:（1）用MTT检测不同浓度的NocturnosideB对人肝癌细胞BEL-7404的增值的影响，观察细胞生长状态和形态学，平板集落形成试验检测NocturnosideB对细胞集落形成的影响，Hoechst染色，检测Caspase3酶