复制型乙型肝炎病毒载体表达病毒白细胞介素10建立小鼠感染模型

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1、授予单位代码10089学号或申请号20102240硕士学位论文科学学位复制型乙型肝炎病毒载体表达病毒白细胞介素10建立小鼠感染模型作者姓名夏欢导师孙殿兴教授专业内科学二级学院白求恩国际和平医院研究起止日期2011年7月—2012年12月交稿日期2013年3月万方数据万方数据目录中文摘要······································································1英文摘要······································································5英文缩写····

2、··································································9研究论文复制型乙型肝炎病毒载体表达病毒白细胞介素10建立小鼠感染模型引言····································································11第一部分表达cmvIL-10、ebvIL-10的复制型HBV载体的构建前言····································································13材料与方法············

3、···············································15结果····································································31附图····································································33讨论····································································37参考文献····························

4、··································38第二部分利用水动力学方法制备重组HBV小鼠感染模型前言····································································42材料与方法···························································44结果····································································44附图························

5、············································46讨论····································································49参考文献······························································51结论··········································································53综述病毒编码的IL-10类似物及其对宿主免疫功能的控制···54致

6、谢··········································································62个人简历····································································63万方数据中文摘要复制型乙型肝炎病毒载体表达病毒白细胞介素10建立小鼠感染模型摘要自从1963年Blumberg发现澳大利亚抗原以来，乙型肝炎病毒（HBV）感染一直是严重威胁人类健康的原因之一。慢性HBV感染所引起的慢性乙型病毒性肝炎及其相关疾病(如肝衰竭、肝硬化、肝癌等)是严重危害人类健康的

7、疾病。目前尚无可靠的HBV动物模型，严重阻碍了HBV相关基础和临床研究。因此，建立易获取、经济适用、生物学背景清晰的小动物HBV感染模型尤为重要。小鼠是医学研究领域常用的实验动物，但是，乙肝病毒的种属特异性使得正常情况下HBV不能感染小鼠。HBV转基因小鼠模型、人鼠嵌合小鼠HBV模型目前己经在一定范围内得到应用，但是这些模型的制备技术要求较高、制备难度大、免疫缺陷和肝细胞来源困难等因素也限制了其应用。水动力学方法建立的HBV转染小鼠