凋亡素2配体对食管癌细胞株eca-109放射敏感性的影响

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1、凋亡素2配体对食管癌细胞株Eca-109放射敏感性的影响摘要目的研究凋亡素2配体(Apo一2ligand，Ap02L)，又称肿瘤坏死因子体(tumornecrosisfactor—relatedapoptosisinducingligand，食管癌细胞株Eca-109放射敏感性的影响。方法MTT法检i贝!JTRAIL对Eca—i09细胞的毒性；克隆形成实验检测TRAIL对Eca一109细胞的放射增敏作用；流式细胞仪(FCM)技术分析TRAIL对凋亡率及细胞周期的影响。结果100～800Hg／mlTRAIL随浓度升高对Eca-109细胞的增殖抑制率增大，药物浓度

2、与细胞抑制率正相关(r=O．981，PO．05)，余联合组的凋亡率均高于单照射组(F=16．97，P

3、40，P

4、sensitivityofApo一2ligand(Ap02L)whichisalsoknownastumornecrosisfactor—relatedapoptosisinducingligand(TRAIL)onesophagealcancercellline．Ecal09invitro．MethodsMTTassayandclonogenicassaywereperformedtoevaluatethecytotoxicityandradiosensitizingeffectofTRAIL，respectively．Thecellapoptosisand

5、cellcycledistributionweremeasuredbyflowcytometry(FCM)．ResultsTheinhibitionofcellgrowthofTRAILwasdosedependentandthe50％inhibitionconcentration(IC50)was637I．tg／m1．TRAILcouldenhancecellradiosensitivityandtheSERWas1．219．Aftertreatedwithdifferentdoseofirradiation．thecellapoptosisofcombin

6、edgroupswerehighercomparedtoirradiationalonegroups，respectively(F=16．97，P

7、clusionsTR触LcouldinhibitEca．109cellgrowthandinducecellapoptosisaswellasGE／Marrest．G2／MarrestmaybeassociatedwiththeradiosensitizationofTRAIL．Postgraduatestudent：Li-LiZhang(Oncology)DirectedbyProf．Xi-GuangLiuKeywords：TRAIL；Esophagealcancercell；Apoptosis；G2／Marrest；Radiosensitivity目录引言

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