茶多酚联合紫杉醇对食管癌eca-109细胞增殖与凋亡的影响

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1、茶多酚联合紫杉醇对食管癌Eca-109细胞增殖与凋亡的影响摘要目的：探讨茶多酚(TP)、紫杉醇(Paclitaxel)抑制食管癌Eca-109细胞生长、诱导细胞凋亡的作用及其可能机制。方法：取对数生长期的Eca-109细胞，分成TP组、Paclitaxel组、TP+Paclitaxel组及对照组，应用MTT法检测细胞抑制率，流式细胞仪检测细胞周期和细胞凋亡率的变化，显微镜观察细胞形态的变化，RT-PCR检测细胞凋亡相关调节基因Caspase一3的表达。结果：不同浓度的TP组、Paclitaxel组，细胞增殖抑制率不同，并与药物浓度、作用时

2、间正相关(F1抽=423．75、279．39；F24h=153．13、274．87；F4sh=355．95、134．46；P

3、)。与TP组、Paclitaxel组比较，TP+Paclitaxel组诱导Eca-109细胞凋亡率增高(F=446．82；q=16．27、31．64；P

4、刘圆圆(肿瘤学)指导教师王秀美教授关键词：茶多酚；紫杉醇；食管癌细胞；细胞凋亡；Caspase一3EffectofTeapolyphenolsandPaclitaxeionproliferationandapoptosisofesophagealcarcinomacelllineEca-109AbstractOBJECTIVEToexploretheeffectsofTeapolyphenolsandpaclitaxelforinhibitingesophagealcancerEca-109cellgrowth，inducingapopt

5、osisandmechanisminvolved．METHODSThelogphaseHL一60cellswerecollected，anddividedintoTP—group，Paclitaxel-group，TP+Paclitaxel—group，andcontrolgroup．CellsurvivalwasobservedbyMTTassay，morphoiogicchangeswereviewedunderlightmicroscopeandcellcycleandcellapoptosiswereoverviewedbyflo

6、wcytometry,andtheexpressionofCaspase-3mRNAbyRT-PCR(ReverseTranscription—PolymeraseChainReaction)．RESULTSCellproliferationinhibitionrateofEta-109cellsindifferent-concentrationTporPaclitaxelwasdifferent，andrelatedtoconcentration,time．TheinhibitionwasmoreremarkablyinTP+Pacli

7、taxgroupsthaninseperategroup．Compared、)I，imthecontrolgroup，TeapolyphenolsinducedG1phaseimprovedremarkably；butPaclitaxel，aloneorincombinationfor24h,inducedG2／MphaseofEca-109cells，improvedremarkably．TheTP+Paclitaxelgroupschangedmoreremarkablythantheseperategroup．Compared、析m

8、theseperategroup，theCaspase一3mRNAexpressionWasimprovedthantheEta-109cellstreatedwiththecombinati